› 5ik3

X-ray diffraction
1.65Å resolution

Crystal structure of anti-gliadin 1002-1E03 Fab fragment

Released:
DOI: 10.2210/pdb5ik3/pdb
PDB entry 5ik3 coloured by chain, front view.
PDB entry 5ik3 coloured by chain, side view.
PDB entry 5ik3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stereotyped antibody responses target posttranslationally modified gluten in celiac disease.
Snir O, Chen X, Gidoni M, du Pré MF, Zhao Y, Steinsbø Ø, Lundin KE, Yaari G, Sollid LM
JCI Insight 2 (2017)
PMID: 28878138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235460 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
1E03 Fab fragment heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 228 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
1E03 Fab fragment light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 221 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 77.28Å b: 138.81Å c: 91.72Å
α: 90° β: 108.2° γ: 90°
R-values:
R R work R free
0.198 0.196 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Computational Strategies for Dissecting the High-Dimensional Complexity of Adaptive Immune Repertoires.
Miho et al. (2018)
2 more

1 mention without citation

Stereotyped antibody responses target posttranslationally modified gluten in celiac disease.
Snir et al. (2017)