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Crystal structure of anti-gliadin 1002-1E03 Fab fragment

X-ray diffraction
1.65Å resolution
PDB entry 5ik3 coloured by chain, front view.
PDB entry 5ik3 coloured by chain, side view.
PDB entry 5ik3 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero dimer
Accessible surface area: 19561.34 Å2
Buried surface area: 3962.67 Å2
Dissociation area: 1,862.49 Å2
Dissociation energy (ΔGdiss): 25.66 kcal/mol
Dissociation entropy (TΔSdiss): 13.15 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-235460
Assembly 2
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Assembly Name: IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero dimer
Accessible surface area: 19888.82 Å2
Buried surface area: 4223.79 Å2
Dissociation area: 1,862.32 Å2
Dissociation energy (ΔGdiss): 20.64 kcal/mol
Dissociation entropy (TΔSdiss): 13.19 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-235460

Macromolecules

1E03 Fab fragment heavy chain
Chains: A, C
Length: 228 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
InterPro:
CATH: Immunoglobulins
1E03 Fab fragment heavy chain in PDB entry 5ik3, assembly 1, front view.
1E03 Fab fragment heavy chain in PDB entry 5ik3, assembly 1, side view.
1E03 Fab fragment heavy chain in PDB entry 5ik3, assembly 1, top view.
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1E03 Fab fragment light chain
Chains: B, D
Length: 221 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
InterPro:
CATH: Immunoglobulins
1E03 Fab fragment light chain in PDB entry 5ik3, assembly 1, front view.
1E03 Fab fragment light chain in PDB entry 5ik3, assembly 1, side view.
1E03 Fab fragment light chain in PDB entry 5ik3, assembly 1, top view.
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