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Cryo-EM structure of the MamK filament at 6.5 A

Electron Microscopy
6.5Å resolution
PDB entry 5jyg coloured by chain, front view.
PDB entry 5jyg coloured by chain, side view.
PDB entry 5jyg coloured by chain, top view.
Source organism: Magnetospirillum magneticum AMB-1
Assembly composition:
homo tetradecamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Actin-like protein MamK
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Multimeric state: homo tetradecamer
Accessible surface area: 232851.58 Å2
Buried surface area: 34111.1 Å2
Dissociation area: 10,082.55 Å2
Dissociation energy (ΔGdiss): -146.17 kcal/mol
Dissociation entropy (TΔSdiss): 188.81 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-174121

Macromolecules

Actin-like protein MamK
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 347 amino acids
Theoretical weight: 37.66 KDa
Source organism: Magnetospirillum magneticum AMB-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2W8Q6 (Residues: 1-347; Coverage: 100%)
Gene names: amb0965, mamK
Pfam: MreB/Mbl protein
InterPro:
Actin-like protein MamK in PDB entry 5jyg, assembly 1, front view.
Actin-like protein MamK in PDB entry 5jyg, assembly 1, side view.
Actin-like protein MamK in PDB entry 5jyg, assembly 1, top view.
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