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Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.

<div class='caption-title'>Cryo-EM Maps of 30S PICs</div>
<div class='caption-title'>Movement of the 30S Head Widens the mRNA Entry Channel and Opens the Latch</div>
<div class='caption-title'>Contacts of IF3 with 30S Ribosomal Subunit</div>
Hussain T, Llácer JL, Wimberly BT, Kieft JS, Ramakrishnan V
OpenAccess logo Cell 167 133-144.e13 (2016)
Related entries: 5lmo, 5lmp, 5lmq, 5lmr, 5lms, 5lmt, 5lmu, 5lmv

Cited: 91 times
EuropePMC logo PMID: 27662086

Abstract

In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.

Reviews - 5lmn mentioned but not cited (1)

  1. Translation initiation in mammalian mitochondria- a prokaryotic perspective. Ayyub SA, Varshney U. RNA Biol 17 165-175 (2020)

Articles - 5lmn mentioned but not cited (11)

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Reviews citing this publication (17)

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