Structure analysis

Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1A)

Electron Microscopy
3.55Å resolution
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero 24-mer (preferred)
Entry contents: 22 distinct polypeptide molecules
2 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 24-mer
Accessible surface area: 270204.48 Å2
Buried surface area: 120214.95 Å2
Dissociation area: 1,802.6 Å2
Dissociation energy (ΔGdiss): 11.43 kcal/mol
Dissociation entropy (TΔSdiss): 11.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-250076

Macromolecules

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Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:
CATH:

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Chain: L
Length: 132 amino acids
Theoretical weight: 14.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: O
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
CATH: S15/NS1, RNA-binding

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Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
CATH: S16 Ribosomal Protein; Chain: A;

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Chain: R
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
CATH: Ribosomal protein S18

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Chain: T
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
CATH: Ribosomal protein S20

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Chain: V
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:

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Chain: W
Length: 72 amino acids
Theoretical weight: 8.25 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHR1 (Residues: 1-72; Coverage: 100%)
Gene names: TTHA1669, infA
Pfam: Translation initiation factor 1A / IF-1
InterPro:
CATH: Nucleic acid-binding proteins

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: Messenger RNA
Representative chains: Y
Length: 42 nucleotides
Theoretical weight: 13.47 KDa

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