› 5ltq

X-ray diffraction
2.05Å resolution

Structure of the Yellow Fluorescent Protein lanYFP from Branchiostoma lanceolatum at pH 7.5

Released:
DOI: 10.2210/pdb5ltq/pdb
PDB entry 5ltq coloured by chain, front view.
PDB entry 5ltq coloured by chain, side view.
PDB entry 5ltq coloured by chain, top view.
Source organism: Branchiostoma lanceolatum
Primary publication:
Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution.
Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert GG, Shaner NC, Royant A
Acta Crystallogr D Struct Biol 72 1298-1307 (2016)
PMID: 27917830

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein blFP-Y3 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 267 amino acids
Theoretical weight: 30.33 KDa
Source organism: Branchiostoma lanceolatum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1PNC0 (Residues: 2-219; Coverage: 100%)
Gene name: blFP-Y3
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CL 16 x CL
1 modified residue:
Ligand CR2 16 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 78.66Å b: 197.16Å c: 115.09Å
α: 90° β: 90.45° γ: 90°
R-values:
R R work R free
0.227 0.225 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structure- and mechanism-guided design of single fluorescent protein-based biosensors.
Nasu et al. (2021)
13 other articles

2 mentions without citation

Identification of mNeonGreen as a pH-Dependent, Turn-On Fluorescent Protein Sensor for Chloride.
Tutol et al. (2019)
1 more