5ltq › Citations

Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution.

Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert GG, Shaner NC, Royant A
Acta Crystallogr D Struct Biol 72 1298-1307 (2016)
Related entries: 5ltp, 5ltr

Cited: 16 times
EuropePMC logo PMID: 27917830

Abstract

Until recently, genes coding for homologues of the autofluorescent protein GFP had only been identified in marine organisms from the phyla Cnidaria and Arthropoda. New fluorescent-protein genes have now been found in the phylum Chordata, coding for particularly bright oligomeric fluorescent proteins such as the tetrameric yellow fluorescent protein lanYFP from Branchiostoma lanceolatum. A successful monomerization attempt led to the development of the bright yellow-green fluorescent protein mNeonGreen. The structures of lanYFP and mNeonGreen have been determined and compared in order to rationalize the directed evolution process leading from a bright, tetrameric to a still bright, monomeric fluorescent protein. An unusual discolouration of crystals of mNeonGreen was observed after X-ray data collection, which was investigated using a combination of X-ray crystallography and UV-visible absorption and Raman spectroscopies, revealing the effects of specific radiation damage in the chromophore cavity. It is shown that X-rays rapidly lead to the protonation of the phenolate O atom of the chromophore and to the loss of its planarity at the methylene bridge.

Articles - 5ltq mentioned but not cited (2)

  1. Identification of mNeonGreen as a pH-Dependent, Turn-On Fluorescent Protein Sensor for Chloride. Tutol JN, Kam HC, Dodani SC. Chembiochem 20 1759-1765 (2019)
  2. Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution. Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert GG, Shaner NC, Royant A. Acta Crystallogr D Struct Biol 72 1298-1307 (2016)


Reviews citing this publication (1)

  1. Structure- and mechanism-guided design of single fluorescent protein-based biosensors. Nasu Y, Shen Y, Kramer L, Campbell RE. Nat Chem Biol 17 509-518 (2021)

Articles citing this publication (13)

  1. High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription. Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL. Cell Syst 8 212-225.e9 (2019)
  2. Specific radiation damage is a lesser concern at room temperature. Gotthard G, Aumonier S, De Sanctis D, Leonard G, von Stetten D, Royant A. IUCrJ 6 665-680 (2019)
  3. Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein. Subach OM, Sotskov VP, Plusnin VV, Gruzdeva AM, Barykina NV, Ivashkina OI, Anokhin KV, Nikolaeva AY, Korzhenevskiy DA, Vlaskina AV, Lazarenko VA, Boyko KM, Rakitina TV, Varizhuk AM, Pozmogova GE, Podgorny OV, Piatkevich KD, Boyden ES, Subach FV. Int J Mol Sci 21 E1644 (2020)
  4. Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen. McCullock TW, MacLean DM, Kammermeier PJ. PLoS One 15 e0219886 (2020)
  5. Photophysical Behavior of mNeonGreen, an Evolutionarily Distant Green Fluorescent Protein. Steiert F, Petrov EP, Schultz P, Schwille P, Weidemann T. Biophys J 114 2419-2431 (2018)
  6. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Hoare SRJ, Tewson PH, Quinn AM, Hughes TE. Sci Rep 10 1766 (2020)
  7. A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride. Tutol JN, Lee J, Chi H, Faizuddin FN, Abeyrathna SS, Zhou Q, Morcos F, Meloni G, Dodani SC. Chem Sci 12 5655-5663 (2021)
  8. A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor. Geethakumari AM, Ahmed WS, Rasool S, Fatima A, Nasir Uddin SM, Aouida M, Biswas KH. Commun Chem 5 117 (2022)
  9. A new insight into RecA filament regulation by RecX from the analysis of conformation-specific interactions. Alekseev A, Pobegalov G, Morozova N, Vedyaykin A, Cherevatenko G, Yakimov A, Baitin D, Khodorkovskii M. Elife 11 e78409 (2022)
  10. Cyan fluorescent proteins derived from mNeonGreen. Zarowny L, Clavel D, Johannson R, Duarte K, Depernet H, Dupuy J, Baker H, Brown A, Royant A, Campbell RE. Protein Eng Des Sel 35 gzac004 (2022)
  11. A monomeric StayGold fluorescent protein. Ivorra-Molla E, Akhuli D, McAndrew MBL, Scott W, Kumar L, Palani S, Mishima M, Crow A, Balasubramanian MK. Nat Biotechnol (2023)
  12. Computational Modeling of the Chlamydial Developmental Cycle Reveals a Potential Role for Asymmetric Division. Chiarelli TJ, Grieshaber NA, Appa C, Grieshaber SS. mSystems 8 e0005323 (2023)
  13. Rational Design of the β-Bulge Gate in a Green Fluorescent Protein Accelerates the Kinetics of Sulfate Sensing. Ong WSY, Ji K, Pathiranage V, Maydew C, Baek K, Villones RLE, Meloni G, Walker AR, Dodani SC. Angew Chem Int Ed Engl 62 e202302304 (2023)


Quick links