› 5mfd

X-ray diffraction
2.3Å resolution

Designed armadillo repeat protein YIIIM''6AII in complex with pD_(KR)5

Released:
DOI: 10.2210/pdb5mfd/pdb
Model geometry
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PDB entry 5mfd coloured by chain, front view.
PDB entry 5mfd coloured by chain, side view.
PDB entry 5mfd coloured by chain, top view.
Source organisms:
Primary publication:
Structures of designed armadillo repeat proteins binding to peptides fused to globular domains.
Hansen S, Kiefer JD, Madhurantakam C, Mittl PRE, Plückthun A
Protein Sci 26 1942-1952 (2017)
PMID: 28691351

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-137222 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
YIIIM''6AII Chains: A, C, E, G, I, J, K, L
Molecule details ›
Chains: A, C, E, G, I, J, K, L
Length: 328 amino acids
Theoretical weight: 34.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Capsid decoration protein Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 109 amino acids
Theoretical weight: 11.48 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P03712 (Residues: 21-110; Coverage: 82%)
Gene names: D, lambdap07
Sequence domains: Bacteriophage lambda head decoration protein D
Structure domains: Head decoration protein D

Ligands and Environments

1 bound ligand:
Ligand CA 49 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P63
Unit cell:
a: 194.66Å b: 194.66Å c: 241.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.214
Expression system: Escherichia coli K-12

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Citations

4 citation in other articles

Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts.
Ernst et al. (2019)
3 more

1 mention without citation

Structures of designed armadillo repeat proteins binding to peptides fused to globular domains.
Hansen et al. (2017)

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