Sample information
Author description:
Structure of a spliceosome remodeled for exon ligation
Source organism:
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 7.9 | NP-40 is also called IGEPAL CA-630 |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK III | 100 | 3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging. |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
4.17 Å
