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TUBULIN-MTC-zampanolide complex

X-ray diffraction
2.2Å resolution
PDB entry 5ng1 coloured by chain, front view.
PDB entry 5ng1 coloured by chain, side view.
PDB entry 5ng1 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 79509.45 Å2
Buried surface area: 22819.68 Å2
Dissociation area: 1,231.74 Å2
Dissociation energy (ΔGdiss): -7.24 kcal/mol
Dissociation entropy (TΔSdiss): 14.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-237077

Macromolecules

Tubulin alpha-1B chain
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Pfam:
InterPro:
CATH:
Tubulin alpha-1B chain in PDB entry 5ng1, assembly 1, front view.
Tubulin alpha-1B chain in PDB entry 5ng1, assembly 1, side view.
Tubulin alpha-1B chain in PDB entry 5ng1, assembly 1, top view.
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Tubulin beta-2B chain
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Pfam:
InterPro:
CATH:
Tubulin beta-2B chain in PDB entry 5ng1, assembly 1, front view.
Tubulin beta-2B chain in PDB entry 5ng1, assembly 1, side view.
Tubulin beta-2B chain in PDB entry 5ng1, assembly 1, top view.
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Stathmin-4
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Pfam: Stathmin family
InterPro:
CATH: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Stathmin-4 in PDB entry 5ng1, assembly 1, front view.
Stathmin-4 in PDB entry 5ng1, assembly 1, side view.
Stathmin-4 in PDB entry 5ng1, assembly 1, top view.
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Tubulin-Tyrosine Ligase
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
InterPro: Tubulin-tyrosine ligase/Tubulin polyglutamylase
CATH:
Tubulin-Tyrosine Ligase in PDB entry 5ng1, assembly 1, front view.
Tubulin-Tyrosine Ligase in PDB entry 5ng1, assembly 1, side view.
Tubulin-Tyrosine Ligase in PDB entry 5ng1, assembly 1, top view.
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