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The structure of the COPI coat leaf

Electron Microscopy
9.2Å resolution
PDB entry 5nzr coloured by chain, front view.
PDB entry 5nzr coloured by chain, side view.
PDB entry 5nzr coloured by chain, top view.
Source organisms:
Assembly composition:
hetero undecamer (preferred)
Entry contents: 7 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero undecamer
PDBe Complex ID: PDB-CPX-129541

Macromolecules

Coatomer subunit alpha
Chain: A
Length: 1262 amino acids
Theoretical weight: 142.53 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8CIE6 (Residues: 1-1224; Coverage: 100%)
Gene name: Copa
Pfam:
InterPro:
Coatomer subunit alpha in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit alpha in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit alpha in PDB entry 5nzr, assembly 1, top view.
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Coatomer subunit beta
Chain: B
Length: 968 amino acids
Theoretical weight: 109.15 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9JIF7 (Residues: 1-953; Coverage: 100%)
Gene names: Copb, Copb1
Pfam:
InterPro:
Coatomer subunit beta in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit beta in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit beta in PDB entry 5nzr, assembly 1, top view.
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Coatomer subunit beta'
Chain: C
Length: 905 amino acids
Theoretical weight: 102.57 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O55029 (Residues: 1-905; Coverage: 100%)
Gene name: Copb2
Pfam:
InterPro:
Coatomer subunit beta' in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit beta' in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit beta' in PDB entry 5nzr, assembly 1, top view.
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Coatomer subunit delta
Chain: D
Length: 511 amino acids
Theoretical weight: 57.3 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5XJY5 (Residues: 1-511; Coverage: 100%)
Gene names: Arcn1, Copd
Pfam:
InterPro:
Coatomer subunit delta in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit delta in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit delta in PDB entry 5nzr, assembly 1, top view.
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ADP-ribosylation factor 1
Chains: F, M, R
Length: 181 amino acids
Theoretical weight: 20.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11076 (Residues: 1-181; Coverage: 100%)
Gene names: ARF1, D1244, YDL192W
Pfam: ADP-ribosylation factor family
InterPro:
ADP-ribosylation factor 1 in PDB entry 5nzr, assembly 1, front view.
ADP-ribosylation factor 1 in PDB entry 5nzr, assembly 1, side view.
ADP-ribosylation factor 1 in PDB entry 5nzr, assembly 1, top view.
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Coatomer subunit gamma-1
Chains: G, K
Length: 874 amino acids
Theoretical weight: 97.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9QZE5 (Residues: 1-874; Coverage: 100%)
Gene names: Copg, Copg1
Pfam:
InterPro:
Coatomer subunit gamma-1 in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit gamma-1 in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit gamma-1 in PDB entry 5nzr, assembly 1, top view.
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Coatomer subunit zeta-1
Chains: L, Z
Length: 177 amino acids
Theoretical weight: 20.22 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61924 (Residues: 1-177; Coverage: 100%)
Gene names: Copz, Copz1
Pfam: Clathrin adaptor complex small chain
InterPro:
Coatomer subunit zeta-1 in PDB entry 5nzr, assembly 1, front view.
Coatomer subunit zeta-1 in PDB entry 5nzr, assembly 1, side view.
Coatomer subunit zeta-1 in PDB entry 5nzr, assembly 1, top view.
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