5o02

X-ray diffraction
1.72Å resolution

GII.17 Kawasaki323 protruding domain in complex with Nanobody Nano-4

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-207248 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.09 KDa
Source organism: Norovirus 13-BH-1/2013/GII.17
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0A7CJV4 (Residues: 225-528; Coverage: 57%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:
Nanobody (VHH) Nano-4 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.59 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2
Unit cell:
a: 118.47Å b: 64.04Å c: 70.03Å
α: 90° β: 97.26° γ: 90°
R-values:
R R work R free
0.152 0.15 0.18
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli