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Cryo-EM structure of jasplakinolide-stabilized malaria parasite F-actin at near-atomic resolution

Electron Microscopy
3.8Å resolution
PDB entry 5ogw coloured by chain, front view.
PDB entry 5ogw coloured by chain, side view.
PDB entry 5ogw coloured by chain, top view.
Source organism: Plasmodium falciparum HB3
Assembly composition:
homo pentamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Actin-1
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Multimeric state: homo pentamer
Accessible surface area: 74175.41 Å2
Buried surface area: 16605.3 Å2
Dissociation area: 2,367.56 Å2
Dissociation energy (ΔGdiss): 0.63 kcal/mol
Dissociation entropy (TΔSdiss): 16.49 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184679

Macromolecules

Actin-1
Chains: A, B, C, D, E
Length: 378 amino acids
Theoretical weight: 42.05 KDa
Source organism: Plasmodium falciparum HB3
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8I4X0 (Residues: 1-376; Coverage: 100%)
Gene names: ACT1, ACTI, PF3D7_1246200, PFL2215w
Pfam: Actin
InterPro:
CATH:
Actin-1 in PDB entry 5ogw, assembly 1, front view.
Actin-1 in PDB entry 5ogw, assembly 1, side view.
Actin-1 in PDB entry 5ogw, assembly 1, top view.
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