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Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) in complex with serine and glycerol

X-ray diffraction
1.68Å resolution
PDB entry 5oi0 coloured by chain, front view.
PDB entry 5oi0 coloured by chain, side view.
PDB entry 5oi0 coloured by chain, top view.
Source organism: Mycolicibacterium hassiacum DSM 44199
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucosylglycerate hydrolase
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Multimeric state: homo tetramer
Accessible surface area: 59465.63 Å2
Buried surface area: 15035.86 Å2
Dissociation area: 4,603.61 Å2
Dissociation energy (ΔGdiss): 0.69 kcal/mol
Dissociation entropy (TΔSdiss): 44.54 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-125567

Macromolecules

Glucosylglycerate hydrolase
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.96 KDa
Source organism: Mycolicibacterium hassiacum DSM 44199
Expression system: Escherichia coli
UniProt:
  • Canonical: K5BDL0 (Residues: 1-446; Coverage: 100%)
Gene names: C731_0006, ggh
Pfam: Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain
InterPro:
Glucosylglycerate hydrolase in PDB entry 5oi0, assembly 1, front view.
Glucosylglycerate hydrolase in PDB entry 5oi0, assembly 1, side view.
Glucosylglycerate hydrolase in PDB entry 5oi0, assembly 1, top view.
PDBe-KB: UniProt Coverage View: K5BDL0  
144850100150200250300350400
 
200400
UniProt
K5BDL0
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Sequence conservation
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