› 5ovj

X-ray diffraction
1.7Å resolution

Structure of the apo form of Mycobacterium smegmatis MabA

Released:
DOI: 10.2210/pdb5ovj/pdb
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PDB entry 5ovj coloured by chain, front view.
PDB entry 5ovj coloured by chain, side view.
PDB entry 5ovj coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural rearrangements occurring upon cofactor binding in the Mycobacterium smegmatis β-ketoacyl-acyl carrier protein reductase MabA.
Küssau T, Flipo M, Van Wyk N, Viljoen A, Olieric V, Kremer L, Blaise M
Acta Crystallogr D Struct Biol 74 383-393 (2018)
PMID: 29717709

Function and Biology Details

Reactions catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase MabA Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 26.73 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71534 (Residues: 1-255; Coverage: 100%)
Gene names: MSMEG_3150, MSMEI_3069, fabG, mabA
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 96.88Å b: 68.27Å c: 71.71Å
α: 90° β: 123.77° γ: 90°
R-values:
R R work R free
0.162 0.16 0.202
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Antimycobacterial activity and molecular docking of methanolic extracts and compounds of marine fungi from Saldanha and False Bays, South Africa.
Tapfuma et al. (2022)

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