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High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule

Electron Microscopy
3.5Å resolution
PDB entry 5syf coloured by chain, front view.
PDB entry 5syf coloured by chain, side view.
PDB entry 5syf coloured by chain, top view.
Source organism: Sus scrofa
Assembly composition:
hetero 52-mer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Tubulin alpha-1B chain and Tubulin beta chain
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Multimeric state: hetero 52-mer
Accessible surface area: 708832.3 Å2
Buried surface area: 275163.04 Å2
Dissociation area: 22,232.73 Å2
Dissociation energy (ΔGdiss): -139.8 kcal/mol
Dissociation entropy (TΔSdiss): 223.15 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135920

Macromolecules

Tubulin alpha-1B chain
Chain: A
Length: 437 amino acids
Theoretical weight: 48.68 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-437; Coverage: 97%)
Gene name: TUBA1B
Pfam:
InterPro:
CATH:
Tubulin alpha-1B chain in PDB entry 5syf, assembly 1, front view.
Tubulin alpha-1B chain in PDB entry 5syf, assembly 1, side view.
Tubulin alpha-1B chain in PDB entry 5syf, assembly 1, top view.
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Tubulin beta chain
Chain: B
Length: 426 amino acids
Theoretical weight: 47.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-426; Coverage: 96%)
Pfam:
InterPro:
CATH:
Tubulin beta chain in PDB entry 5syf, assembly 1, front view.
Tubulin beta chain in PDB entry 5syf, assembly 1, side view.
Tubulin beta chain in PDB entry 5syf, assembly 1, top view.
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