› 5tre

Electron Microscopy
15.6Å resolution

Zinc and the Iron Donor Frataxin Regulate Oligomerization of the Scaffold Protein to Form New Fe-S Cluster Assembly Centers

Released:
DOI: 10.2210/pdb5tre/pdb
PDB entry 5tre coloured by chain, front view.
PDB entry 5tre coloured by chain, side view.
PDB entry 5tre coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Zinc and the iron donor frataxin regulate oligomerization of the scaffold protein to form new Fe-S cluster assembly centers.
Galeano BK, Ranatunga W, Gakh O, Smith DY, Thompson JR, Isaya G
OpenAccess logo Metallomics 9 773-801 (2017)
PMID: 28548666
Related structures: EMD-8458

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Iron sulfur cluster assembly protein 1, mitochondrial Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x
Length: 142 amino acids
Theoretical weight: 15.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: Q03020 (Residues: 28-165; Coverage: 84%)
Gene names: ISU1, NUA1, YPL135W
Sequence domains: NifU-like N terminal domain
Frataxin homolog, mitochondrial Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 121 amino acids
Theoretical weight: 13.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: Q07540 (Residues: 52-172; Coverage: 70%)
Gene names: YDL120W, YFH1
Sequence domains: Frataxin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 15.6Å
Relevant EMDB volumes: EMD-8458
Expression system: Escherichia coli #1/H766

Quick links

Citations

2 review citations

Iron-sulfur cluster biosynthesis and trafficking - impact on human disease conditions.
Wachnowsky et al. (2018)
1 more

1 mention without citation

Zinc and the iron donor frataxin regulate oligomerization of the scaffold protein to form new Fe-S cluster assembly centers.
Galeano et al. (2017)