› 5uky

X-ray diffraction
2.02Å resolution

DHp domain of PhoR of M. tuberculosis - native data

Released:
DOI: 10.2210/pdb5uky/pdb
PDB entry 5uky coloured by chain, front view.
PDB entry 5uky coloured by chain, side view.
PDB entry 5uky coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Asymmetric Structure of the Dimerization Domain of PhoR, a Sensor Kinase Important for the Virulence of Mycobacterium tuberculosis.
Xing D, Ryndak MB, Wang L, Kolesnikova I, Smith I, Wang S
OpenAccess logo ACS Omega 2 3509-3517 (2017)
PMID: 28782049

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 amino acids
Theoretical weight: 8.36 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71815 (Residues: 240-310; Coverage: 15%)
Gene names: Rv0758, phoR
Sequence domains: His Kinase A (phospho-acceptor) domain

Ligands and Environments

5 bound ligands:
Ligand K 1 x K
Ligand EDO 3 x EDO
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 23.25Å b: 74.534Å c: 44.539Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.173 0.171 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Two-component sensor histidine kinases of Mycobacterium tuberculosis: Beacons for niche navigation.
Stupar et al. (2022)
6 other articles

1 mention without citation

Natural mutations in the sensor kinase of the PhoPR two-component regulatory system modulate virulence of ancestor-like tuberculosis bacilli.
Malaga et al. (2023)