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Crystal structure of BhGH81 mutant in complex with laminaro-pentaose

X-ray diffraction
1.7Å resolution
PDB entry 5upo coloured by chain, front view.
PDB entry 5upo coloured by chain, side view.
PDB entry 5upo coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucan endo-1,3-beta-D-glucosidase
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Multimeric state: monomeric
Accessible surface area: 24844.46 Å2
Buried surface area: 6529.48 Å2
Dissociation area: 81.01 Å2
Dissociation energy (ΔGdiss): -1.53 kcal/mol
Dissociation entropy (TΔSdiss): -0.34 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191874

Macromolecules

Glucan endo-1,3-beta-D-glucosidase
Chain: A
Length: 753 amino acids
Theoretical weight: 85.28 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KG76 (Residues: 28-779; Coverage: 76%)
Gene name: BH0236
Pfam:
InterPro:
Glucan endo-1,3-beta-D-glucosidase in PDB entry 5upo, assembly 1, front view.
Glucan endo-1,3-beta-D-glucosidase in PDB entry 5upo, assembly 1, side view.
Glucan endo-1,3-beta-D-glucosidase in PDB entry 5upo, assembly 1, top view.
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