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Crystal structure of C-As lyase with mutation K105A and substrate Roxarsone

X-ray diffraction
2.23Å resolution
PDB entry 5v0f coloured by chain, front view.
PDB entry 5v0f coloured by chain, side view.
PDB entry 5v0f coloured by chain, top view.
Source organism: Thermomonospora curvata DSM 43183
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: VOC domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 6589.26 Å2
Buried surface area: 255.62 Å2
Dissociation area: 46.24 Å2
Dissociation energy (ΔGdiss): 6.9 kcal/mol
Dissociation entropy (TΔSdiss): -0.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112226

Macromolecules

VOC domain-containing protein
Chain: A
Length: 126 amino acids
Theoretical weight: 13.39 KDa
Source organism: Thermomonospora curvata DSM 43183
Expression system: Escherichia coli
UniProt:
  • Canonical: D1A230 (Residues: 1-123; Coverage: 82%)
Gene name: Tcur_4156
Pfam: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
InterPro:
CATH: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1
VOC domain-containing protein in PDB entry 5v0f, assembly 1, front view.
VOC domain-containing protein in PDB entry 5v0f, assembly 1, side view.
VOC domain-containing protein in PDB entry 5v0f, assembly 1, top view.
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