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Post-catalytic complex of human Polymerase Mu (G433A) mutant with incoming dTTP

X-ray diffraction
1.65Å resolution
PDB entry 5vz9 coloured by chain, front view.
PDB entry 5vz9 coloured by chain, side view.
PDB entry 5vz9 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
3 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-directed DNA/RNA polymerase mu and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 15838.61 Å2
Buried surface area: 5777.65 Å2
Dissociation area: 575.87 Å2
Dissociation energy (ΔGdiss): 8.57 kcal/mol
Dissociation entropy (TΔSdiss): 7.43 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-285834

Macromolecules

DNA-directed DNA/RNA polymerase mu
Chain: A
Length: 354 amino acids
Theoretical weight: 39.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 134-397, 411-494; Coverage: 70%)
Gene names: POLM, polmu
Pfam:
InterPro:
CATH:
DNA-directed DNA/RNA polymerase mu in PDB entry 5vz9, assembly 1, front view.
DNA-directed DNA/RNA polymerase mu in PDB entry 5vz9, assembly 1, side view.
DNA-directed DNA/RNA polymerase mu in PDB entry 5vz9, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q9NP87  
135450100150200250300350
 
100200300GSAAASPAWMPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQTMKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDLSTPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLITHPKEGQEAGLLPRVMCRLQDQGLILYHQHQHSCCESPTRLAQQSHMDAFERSFCIFRLPQPGSWKAVRVDLVVAPVSQFPFALLAWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTFFQAASEEDIFRHLGLEYLPPEQRNA
UniProt
Q9NP87
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Sequence conservation
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DNA chain T
Name: DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')
Representative chains: T
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5vz9, assembly 1, front view.
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5vz9, assembly 1, side view.
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5vz9, assembly 1, top view.
19123456789
 
246810CGGCATACG
Chains
Chain B (auth T)
Ligand binding sites
Interaction interfaces
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DNA chain P
Name: DNA (5'-D(*CP*GP*TP*AP*T)-3')
Representative chains: P
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5vz9, assembly 1, front view.
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5vz9, assembly 1, side view.
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5vz9, assembly 1, top view.
151.01.52.02.53.03.54.04.55.0
 
246CGTAT
Chains
Chain C (auth P)
Ligand binding sites
Interaction interfaces
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DNA chain D
Name: DNA (5'-D(P*GP*CP*CP*G)-3')
Representative chains: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5vz9, assembly 1, front view.
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5vz9, assembly 1, side view.
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5vz9, assembly 1, top view.
141.01.21.41.61.82.02.22.42.62.83.03.23.43.63.84.0
 
12345GCCG
Chains
Chain D (auth D)
Interaction interfaces
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