5wk2 › Citations

Structural insights into chemokine CCL17 recognition by antibody M116.
Fig. 1
Fig. 2
Teplyakov A, Obmolova G, Gilliland GL
OpenAccess logo Biochem Biophys Rep 13 27-31 (2018)
Cited: 6 times
EuropePMC logo PMID: 29264403

Abstract

The homeostatic chemokine CCL17, also known as thymus and activation regulated chemokine (TARC), has been associated with various diseases such as asthma, idiopathic pulmonary fibrosis, atopic dermatitis and ulcerative colitis. Neutralization of CCL17 by antibody treatment ameliorates the impact of disease by blocking influx of T cells. Monoclonal antibody M116 derived from a combinatorial library shows potency in neutralizing CCL17-induced signaling. To gain insight into the structural determinants of antigen recognition, the crystal structure of M116 Fab was determined in complex with CCL17 and in the unbound form. Comparison of the structures revealed an unusual induced-fit mechanism of antigen recognition that involves cis-trans isomerization in two CDRs. The structure of the CCL17-M116 complex revealed the antibody binding epitope, which does not overlap with the putative receptor epitope, suggesting that the current model of chemokine-receptor interactions, as observed in the CXCR4-vMIP-II system, may not be universal.

Articles - 5wk2 mentioned but not cited (3)

  1. An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants. Guest JD, Vreven T, Zhou J, Moal I, Jeliazkov JR, Gray JJ, Weng Z, Pierce BG. Structure 29 606-621.e5 (2021)
  2. Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides. Rahumatullah A, Balachandra D, Noordin R, Baharudeen Z, Lim YY, Choong YS, Lim TS. Sci Rep 11 2502 (2021)
  3. Structural insights into chemokine CCL17 recognition by antibody M116. Teplyakov A, Obmolova G, Gilliland GL. Biochem Biophys Rep 13 27-31 (2018)


Reviews citing this publication (1)

  1. Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review). Berghi NO, Dumitru M, Vrinceanu D, Ciuluvica RC, Simioniuc-Petrescu A, Caragheorgheopol R, Tucureanu C, Cornateanu RS, Giurcaneanu C. Exp Ther Med 20 2352-2360 (2020)

Articles citing this publication (2)

  1. Chemokine Expression-Based Endotype Clustering of Chronic Rhinosinusitis. Förster-Ruhrmann U, Szczepek AJ, Pierchalla G, Fluhr JW, Artuc M, Zuberbier T, Bachert C, Olze H. J Pers Med 12 646 (2022)
  2. Elucidating the Molecular Interactions of Chemokine CCL2 Orthologs with Flavonoid Baicalin. Joshi N, Kumar D, Poluri KM. ACS Omega 5 22637-22651 (2020)


Quick links