› 5wnb

X-ray diffraction
2.4Å resolution

Structure of antibody 3D3 bound to the linear epitope of RSV G

Released:
DOI: 10.2210/pdb5wnb/pdb
PDB entry 5wnb coloured by chain, front view.
PDB entry 5wnb coloured by chain, side view.
PDB entry 5wnb coloured by chain, top view.
Source organisms:
Primary publication:
Structures of respiratory syncytial virus G antigen bound to broadly neutralizing antibodies.
Fedechkin SO, George NL, Wolff JT, Kauvar LM, DuBois RM
Sci Immunol 3 (2018)
PMID: 29523582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230775 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
mAb 3D3 Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 231 amino acids
Theoretical weight: 24.78 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
mAb 3D3 Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Mature secreted glycoprotein G Chains: A, B
Molecule details ›
Chains: A, B
Length: 11 amino acids
Theoretical weight: 1.4 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Not provided
UniProt:
  • Canonical: P03423 (Residues: 162-172; Coverage: 4%)
Gene name: G

Ligands and Environments

2 bound ligands:
Ligand ZN 14 x ZN
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 68.76Å b: 105.43Å c: 121.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.268
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

13 review citations

Fc-Mediated Antibody Effector Functions During Respiratory Syncytial Virus Infection and Disease.
van Erp et al. (2019)
12 more

1 mention without citation

Structures of respiratory syncytial virus G antigen bound to broadly neutralizing antibodies.
Fedechkin et al. (2018)