Structure analysis

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

X-ray diffraction
3.4Å resolution
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero 23-mer (preferred)
Entry contents: 20 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 23-mer
Accessible surface area: 273589.0 Å2
Buried surface area: 135733.89 Å2
Dissociation area: 649.36 Å2
Dissociation energy (ΔGdiss): 4.88 kcal/mol
Dissociation entropy (TΔSdiss): 9.45 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215921

Macromolecules

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Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:
CATH: Dna Ligase; domain 1

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Chain: L
Length: 125 amino acids
Theoretical weight: 13.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 2-126; Coverage: 95%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: O
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 2-89; Coverage: 99%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chain: P
Length: 84 amino acids
Theoretical weight: 10 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-84; Coverage: 96%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chain: Q
Length: 99 amino acids
Theoretical weight: 11.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 2-100; Coverage: 94%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
InterPro:

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Chain: R
Length: 73 amino acids
Theoretical weight: 8.5 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 16-88; Coverage: 83%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chain: T
Length: 99 amino acids
Theoretical weight: 10.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 8-106; Coverage: 93%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
CATH: Ribosomal protein S20

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Chain: U
Length: 25 amino acids
Theoretical weight: 3.09 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 2-26; Coverage: 93%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:

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Name: 16S Ribosomal RNA rRNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 494.17 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: RNA (5'-R(*AP*(A2M)P*A)-3')
Representative chains: a
Length: 3 nucleotides
Theoretical weight: 957 Da

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Name: RNA (5'-R(P*UP*(70U)P*UP*UP*(12A)P*A)-3')
Representative chains: b
Length: 6 nucleotides
Theoretical weight: 2.12 KDa

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