› 5wyg

X-ray diffraction
2.36Å resolution

The crystal structure of the apo form of Mtb MazF

Released:
DOI: 10.2210/pdb5wyg/pdb
PDB entry 5wyg coloured by chain, front view.
PDB entry 5wyg coloured by chain, side view.
PDB entry 5wyg coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis.
Chen R, Tu J, Liu Z, Meng F, Ma P, Ding Z, Yang C, Chen L, Deng X, Xie W
Biochem Biophys Res Commun 486 804-810 (2017)
PMID: 28351618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable endoribonuclease MazF7 Chains: A, C
Molecule details ›
Chains: A, C
Length: 140 amino acids
Theoretical weight: 14.65 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL62 (Residues: 1-136; Coverage: 100%)
Gene names: Rv2063A, mazF7
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P65
Unit cell:
a: 74.468Å b: 74.468Å c: 105.114Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.224
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.
Jurėnas et al. (2020)

6 mentions without citation

Translational regulation in mycobacteria and its implications for pathogenicity.
Sawyer et al. (2018)
5 more