Function and Biology

Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-homocysteine intermediates.

Source organism: Pseudomonas putida
Biochemical function: homocysteine desulfhydrase activity
Biological process: transsulfuration
Cellular component: cytoplasm

EC 4.4.1.2: Homocysteine desulfhydrase

Reaction catalysed:
(1a) L-homocysteine = hydrogen sulfide + 2-aminobut-2-enoate
Systematic name:
L-homocysteine hydrogen-sulfide-lyase (deaminating; 2-oxobutanoate-forming)
Alternative Name(s):
  • Homocysteine desulfurase
  • L-homocysteine hydrogen-sulfide-lyase (deaminating)

EC 4.4.1.11: Methionine gamma-lyase

Reaction catalysed:
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Systematic name:
L-methionine methanethiol-lyase (deaminating; 2-oxobutanoate-forming)
Alternative Name(s):
  • L-methioninase
  • L-methionine gamma-lyase
  • L-methionine methanethiol-lyase (deaminating)
  • Methioninase
  • Methionine dethiomethylase
  • Methionine lyase

Sequence family

Pfam Protein family (Pfam)
PF01053
Domain description: Cys/Met metabolism PLP-dependent enzyme
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase
The deposited structure of PDB entry 5x30 contains 2 copies of Pfam domain PF01053 (Cys/Met metabolism PLP-dependent enzyme) in L-methionine gamma-lyase. Showing 1 copy in chain C.
The deposited structure of PDB entry 5x30 contains 2 copies of Pfam domain PF01053 (Cys/Met metabolism PLP-dependent enzyme) in L-methionine gamma-lyase. Showing 1 copy in chain D.

InterPro InterPro annotations
IPR000277
Domain description: Cys/Met metabolism, pyridoxal phosphate-dependent enzyme
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase
IPR015422
Domain description: Pyridoxal phosphate-dependent transferase, small domain
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase
IPR015424
Domain description: Pyridoxal phosphate-dependent transferase
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase
IPR006237
Domain description: L-methionine gamma-lyase
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase
IPR015421
Domain description: Pyridoxal phosphate-dependent transferase, major domain
Occurring in:
  1. L-methionine gamma-lyase
  2. L-methionine gamma-lyase