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Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-homocysteine intermediates.

X-ray diffraction
1.7Å resolution
PDB entry 5x30 coloured by chain, front view.
PDB entry 5x30 coloured by chain, side view.
PDB entry 5x30 coloured by chain, top view.
Source organism: Pseudomonas putida
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: L-methionine gamma-lyase
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Multimeric state: hetero tetramer
Accessible surface area: 46783.37 Å2
Buried surface area: 19534.61 Å2
Dissociation area: 4,427.2 Å2
Dissociation energy (ΔGdiss): 55.05 kcal/mol
Dissociation entropy (TΔSdiss): 16.05 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-146525

Macromolecules

L-methionine gamma-lyase
Chains: A, C
Length: 398 amino acids
Theoretical weight: 42.94 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P13254 (Residues: 1-398; Coverage: 100%)
Gene name: mdeA
Pfam: Cys/Met metabolism PLP-dependent enzyme
InterPro:
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, front view.
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, side view.
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, top view.
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L-methionine gamma-lyase
Chains: B, D
Length: 398 amino acids
Theoretical weight: 42.71 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P13254 (Residues: 1-398; Coverage: 100%)
Gene name: mdeA
Pfam: Cys/Met metabolism PLP-dependent enzyme
InterPro:
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, front view.
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, side view.
L-methionine gamma-lyase in PDB entry 5x30, assembly 1, top view.
Molecule details ›

Search similar proteins

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