5y6r

X-ray diffraction
3.01Å resolution

Crystal structure of CSFV NS5B

Released:

Function and Biology Details

Reactions catalysed:
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Genome polyprotein Chain: A
Molecule details ›
Chain: A
Length: 695 amino acids
Theoretical weight: 79.68 KDa
Source organism: Classical swine fever virus strain Eystrup
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99BK1 (Residues: 3181-3874; Coverage: 18%)
Sequence domains: Viral RNA dependent RNA polymerase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P4332
Unit cell:
a: 229.99Å b: 229.99Å c: 229.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.236
Expression system: Escherichia coli