5y6r Citations

Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain.

J Virol 92 (2018)
Cited: 16 times
EuropePMC logo PMID: 29720518

Abstract

Classical swine fever virus (CSFV) is the cause of classical swine fever (CSF). Nonstructural protein 5B (NS5B) is an RNA-dependent RNA polymerase (RdRp) that is a key enzyme initiating viral RNA replication by a de novo mechanism. It is also an attractive target for the development of anti-CSFV drugs. To gain a better understanding of the mechanism of CSFV RNA synthesis, here, we solved the first crystal structure of CSFV NS5B. Our studies show that the CSFV NS5B RdRp contains the characteristic finger, palm, and thumb domains, as well as a unique N-terminal domain (NTD) that has never been observed. Mutagenesis studies on NS5B validated the importance of the NTD in the catalytic activity of this novel RNA-dependent RNA polymerase. Moreover, our results shed light on CSFV infection.

Reviews - 5y6r mentioned but not cited (2)

  1. Atypical Porcine Pestiviruses: Relationships and Conserved Structural Features. Riedel C, Aitkenhead H, El Omari K, Rümenapf T. Viruses 13 760 (2021)
  2. Revisiting Viral RNA-Dependent RNA Polymerases: Insights from Recent Structural Studies. Ramaswamy K, Rashid M, Ramasamy S, Jayavelu T, Venkataraman S. Viruses 14 2200 (2022)

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