› 5yhw

X-ray diffraction
2.7Å resolution

Crystal structure of Pig SAMHD1

Released:
DOI: 10.2210/pdb5yhw/pdb
PDB entry 5yhw coloured by chain, front view.
PDB entry 5yhw coloured by chain, side view.
PDB entry 5yhw coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural characterization and directed modification of Sus scrofa SAMHD1 reveal the mechanism underlying deoxynucleotide regulation.
Kong J, Wang MM, He SY, Peng X, Qin XH
FEBS J 286 3844-3857 (2019)
PMID: 31152619

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 524 amino acids
Theoretical weight: 60.77 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: I3LG77 (Residues: 110-627; Coverage: 83%)
Gene name: SAMHD1
Sequence domains: HD domain

Ligands and Environments

2 bound ligands:
Ligand DGT 24 x DGT
Ligand MG 16 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 86.54Å b: 95.909Å c: 130.749Å
α: 78.66° β: 88.12° γ: 82.68°
R-values:
R R work R free
0.264 0.263 0.29
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

1 mention without citation

Biochemical functions and structure of Caenorhabditis elegans ZK177.8 protein: Aicardi-Goutières syndrome SAMHD1 dNTPase ortholog.
Maehigashi et al. (2023)