Assemblies
Assembly Name:
Exon junction subcomplex MAGOHB-Y14 and RNA
Multimeric state:
hetero 55-mer
Accessible surface area:
480091.62 Å2
Buried surface area:
201529.06 Å2
Dissociation area:
1,213.97
Å2
Dissociation energy (ΔGdiss):
0.86
kcal/mol
Dissociation entropy (TΔSdiss):
9.79
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-249678
Macromolecules
Chain: A
Length: 2335 amino acids
Theoretical weight: 273.97 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
Length: 2335 amino acids
Theoretical weight: 273.97 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q6P2Q9 (Residues: 1-2335; Coverage: 100%)
Pfam:
- PRO8NT (NUC069), PrP8 N-terminal domain
- PROCN (NUC071) domain
- RNA recognition motif of the spliceosomal PrP8
- U5-snRNA binding site 2 of PrP8
- U6-snRNA interacting domain of PrP8
- PRP8 domain IV core
- JAB1/Mov34/MPN/PAD-1 ubiquitin protease
- PROCT (NUC072) domain
- Pre-mRNA-processing-splicing factor 8
- PRO8NT domain
- PROCN domain
- RNA recognition motif, spliceosomal PrP8
- Ribonuclease H-like superfamily
- Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily
- Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding
- Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding
- PRP8 domain IV core
- Prp8 RNase domain IV, palm region
- Prp8 RNase domain IV, fingers region
- JAB1/MPN/MOV34 metalloenzyme domain
- MPN domain
- PROCT domain
Chain: C
Length: 972 amino acids
Theoretical weight: 109.56 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
Length: 972 amino acids
Theoretical weight: 109.56 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q15029 (Residues: 1-972; Coverage: 100%)
Pfam:
- 116 kDa U5 small nuclear ribonucleoprotein component N-terminus
- Elongation factor Tu GTP binding domain
- Elongation factor Tu domain 2
- Elongation Factor G, domain III
- Elongation factor G, domain IV
- Elongation factor G C-terminus
- 116kDa U5 small nuclear ribonucleoprotein component, N-terminal
- P-loop containing nucleoside triphosphate hydrolase
- Translational (tr)-type GTP-binding domain
- Small GTP-binding domain
- Snu114, GTP-binding domain
- Translation protein, beta-barrel domain superfamily
- Translation elongation factor EFTu-like, domain 2
- EF-G domain III/V-like
- Elongation Factor G, domain II
- Ribosomal protein uS5 domain 2-type superfamily
- Small ribosomal subunit protein uS5 domain 2-type fold, subgroup
- Translation elongation factor EFG/EF2, domain IV
- Elongation factor EFG, domain V-like
- 116kDa U5 small nuclear ribonucleoprotein component, C-terminal
Chain: D
Length: 2136 amino acids
Theoretical weight: 244.82 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
Length: 2136 amino acids
Theoretical weight: 244.82 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O75643 (Residues: 1-2136; Coverage: 100%)
Pfam:
- Pre-mRNA-splicing helicase BRR2 plug domain
- N-terminal helicase PWI domain
- DEAD/DEAH box helicase
- Helicase conserved C-terminal domain
- Sec63 Brl domain
- P-loop containing nucleoside triphosphate hydrolase
- Brr2, N-terminal helicase PWI domain
- Helicase superfamily 1/2, ATP-binding domain
- DEAD/DEAH box helicase domain
- Helicase, C-terminal domain-like
- Winged helix DNA-binding domain superfamily
- Winged helix-like DNA-binding domain superfamily
- Sec63 domain
- C2 domain superfamily
- Immunoglobulin E-set
Chain: E
Length: 357 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
UniProt:
Pfam: WD domain, G-beta repeat
InterPro:
Length: 357 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q96DI7 (Residues: 1-357; Coverage: 100%)
Pfam: WD domain, G-beta repeat
InterPro:
Chain: I
Length: 855 amino acids
Theoretical weight: 100.15 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Tetratricopeptide repeat
InterPro:
Length: 855 amino acids
Theoretical weight: 100.15 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9HCS7 (Residues: 1-855; Coverage: 100%)
Pfam: Tetratricopeptide repeat
InterPro:
Chain: J
Length: 848 amino acids
Theoretical weight: 100.61 KDa
Source organism: Homo sapiens
UniProt:
Pfam: HAT (Half-A-TPR) repeat
InterPro:
Length: 848 amino acids
Theoretical weight: 100.61 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9BZJ0 (Residues: 1-848; Coverage: 100%)
Pfam: HAT (Half-A-TPR) repeat
InterPro:
Chain: K
Length: 225 amino acids
Theoretical weight: 26.16 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Breast carcinoma amplified sequence 2 (BCAS2)
InterPro: Pre-mRNA-splicing factor SPF27
Length: 225 amino acids
Theoretical weight: 26.16 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O75934 (Residues: 1-225; Coverage: 100%)
Pfam: Breast carcinoma amplified sequence 2 (BCAS2)
InterPro: Pre-mRNA-splicing factor SPF27
Chain: L
Length: 802 amino acids
Theoretical weight: 92.41 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 802 amino acids
Theoretical weight: 92.41 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q99459 (Residues: 1-802; Coverage: 100%)
Pfam:
InterPro:
Chain: y
Length: 307 amino acids
Theoretical weight: 35.5 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Isy1-like splicing family
InterPro:
Length: 307 amino acids
Theoretical weight: 35.5 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9ULR0 (Residues: 1-139, 140-140, 141-285; Coverage: 100%)
- Best match: Q9ULR0-2 (Residues: 1-307)
Pfam: Isy1-like splicing family
InterPro:
Chain: M
Length: 243 amino acids
Theoretical weight: 28.78 KDa
Source organism: Homo sapiens
UniProt:
Pfam: SYF2 splicing factor
InterPro: mRNA splicing factor SYF2
Length: 243 amino acids
Theoretical weight: 28.78 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O95926 (Residues: 1-243; Coverage: 100%)
Pfam: SYF2 splicing factor
InterPro: mRNA splicing factor SYF2
Chain: N
Length: 144 amino acids
Theoretical weight: 17.03 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Pre-mRNA-splicing factor BUD31
InterPro:
Length: 144 amino acids
Theoretical weight: 17.03 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P41223 (Residues: 1-144; Coverage: 100%)
Pfam: Pre-mRNA-splicing factor BUD31
InterPro:
Chain: O
Length: 420 amino acids
Theoretical weight: 46.96 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 420 amino acids
Theoretical weight: 46.96 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9NW64 (Residues: 1-420; Coverage: 100%)
Pfam:
InterPro:
Chain: P
Length: 229 amino acids
Theoretical weight: 26.67 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Cwf15/Cwc15 cell cycle control protein
InterPro: Pre-mRNA-splicing factor Cwf15/Cwc15
Length: 229 amino acids
Theoretical weight: 26.67 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9P013 (Residues: 1-229; Coverage: 100%)
Pfam: Cwf15/Cwc15 cell cycle control protein
InterPro: Pre-mRNA-splicing factor Cwf15/Cwc15
Chain: R
Length: 536 amino acids
Theoretical weight: 61.77 KDa
Source organism: Homo sapiens
UniProt:
Pfam: SKIP/SNW domain
InterPro:
Length: 536 amino acids
Theoretical weight: 61.77 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q13573 (Residues: 1-536; Coverage: 100%)
Pfam: SKIP/SNW domain
InterPro:
Chain: S
Length: 166 amino acids
Theoretical weight: 18.26 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
InterPro:
Length: 166 amino acids
Theoretical weight: 18.26 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9Y3C6 (Residues: 1-166; Coverage: 100%)
Pfam: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
InterPro:
Chain: T
Length: 514 amino acids
Theoretical weight: 57.28 KDa
Source organism: Homo sapiens
UniProt:
Pfam: WD domain, G-beta repeat
InterPro:
Length: 514 amino acids
Theoretical weight: 57.28 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O43660 (Residues: 1-514; Coverage: 100%)
Pfam: WD domain, G-beta repeat
InterPro:
Chain: Q
Length: 1485 amino acids
Theoretical weight: 171.5 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
Length: 1485 amino acids
Theoretical weight: 171.5 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O60306 (Residues: 1-1485; Coverage: 100%)
Pfam:
- Intron-binding protein aquarius N-terminal
- Intron-binding protein aquarius, beta-barrel
- Intron-binding protein aquarius insert domain
- AAA domain
- AAA domain
Chain: U
Length: 2752 amino acids
Theoretical weight: 300.26 KDa
Source organism: Homo sapiens
UniProt:
Pfam: cwf21 domain
InterPro:
Length: 2752 amino acids
Theoretical weight: 300.26 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9UQ35 (Residues: 1-2752; Coverage: 100%)
Pfam: cwf21 domain
InterPro:
Chain: V
Length: 908 amino acids
Theoretical weight: 105.65 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 908 amino acids
Theoretical weight: 105.65 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9HCG8 (Residues: 1-908; Coverage: 100%)
Pfam:
InterPro:
Chain: W
Length: 579 amino acids
Theoretical weight: 65.61 KDa
Source organism: Homo sapiens
UniProt:
Pfam: WD domain, G-beta repeat
InterPro:
Length: 579 amino acids
Theoretical weight: 65.61 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O60508 (Residues: 1-579; Coverage: 100%)
Pfam: WD domain, G-beta repeat
InterPro:
Chain: X
Length: 425 amino acids
Theoretical weight: 49.74 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 425 amino acids
Theoretical weight: 49.74 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9NXE8 (Residues: 1-425; Coverage: 100%)
Pfam:
InterPro:
Chain: Y
Length: 323 amino acids
Theoretical weight: 37.14 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Saf4/Yju2 protein
InterPro:
Length: 323 amino acids
Theoretical weight: 37.14 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9BW85 (Residues: 1-323; Coverage: 100%)
Pfam: Saf4/Yju2 protein
InterPro:
Chain: Z
Length: 1227 amino acids
Theoretical weight: 140.72 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
Length: 1227 amino acids
Theoretical weight: 140.72 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q92620 (Residues: 1-1227; Coverage: 100%)
Pfam:
- DEAD/DEAH box helicase
- Helicase conserved C-terminal domain
- Helicase associated domain (HA2), winged-helix
- Helicase associated domain (HA2), ratchet-like
- Oligonucleotide/oligosaccharide-binding (OB)-fold
Chain: u
Length: 411 amino acids
Theoretical weight: 46.93 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 411 amino acids
Theoretical weight: 46.93 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P38919 (Residues: 1-411; Coverage: 100%)
Pfam:
InterPro:
Chain: v
Length: 148 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Mago nashi protein
InterPro:
Length: 148 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q96A72 (Residues: 1-148; Coverage: 100%)
Pfam: Mago nashi protein
InterPro:
Chain: w
Length: 174 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
UniProt:
Pfam: RNA recognition motif
InterPro:
Length: 174 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9Y5S9 (Residues: 1-174; Coverage: 100%)
Pfam: RNA recognition motif
InterPro:
Chain: x
Length: 703 amino acids
Theoretical weight: 76.38 KDa
Source organism: Homo sapiens
UniProt:
Pfam: CASC3/Barentsz eIF4AIII binding
InterPro:
Length: 703 amino acids
Theoretical weight: 76.38 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: O15234 (Residues: 1-703; Coverage: 100%)
Pfam: CASC3/Barentsz eIF4AIII binding
InterPro:
Chains: a, h
Length: 126 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 126 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62318 (Residues: 1-126; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chains: b, i
Length: 229 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 229 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P14678 (Residues: 1-229; Coverage: 95%)
Pfam: LSM domain
InterPro:
Chains: c, j
Length: 119 amino acids
Theoretical weight: 13.31 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 119 amino acids
Theoretical weight: 13.31 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62314 (Residues: 1-119; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chains: d, k
Length: 118 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 118 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62316 (Residues: 1-118; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chains: f, m
Length: 86 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 86 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62306 (Residues: 1-86; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chains: e, l
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62304 (Residues: 1-92; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chains: g, n
Length: 76 amino acids
Theoretical weight: 8.51 KDa
Source organism: Homo sapiens
UniProt:
Pfam: LSM domain
InterPro:
Length: 76 amino acids
Theoretical weight: 8.51 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P62308 (Residues: 1-76; Coverage: 100%)
Pfam: LSM domain
InterPro:
Chain: o
Length: 255 amino acids
Theoretical weight: 28.46 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Leucine-rich repeat
InterPro:
Length: 255 amino acids
Theoretical weight: 28.46 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P09661 (Residues: 1-255; Coverage: 100%)
Pfam: Leucine-rich repeat
InterPro:
Chain: p
Length: 225 amino acids
Theoretical weight: 25.52 KDa
Source organism: Homo sapiens
UniProt:
Pfam: RNA recognition motif
InterPro:
Length: 225 amino acids
Theoretical weight: 25.52 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P08579 (Residues: 1-225; Coverage: 100%)
Pfam: RNA recognition motif
InterPro:
Chain: 1
Length: 301 amino acids
Theoretical weight: 33.48 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 301 amino acids
Theoretical weight: 33.48 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q9UNP9 (Residues: 1-301; Coverage: 100%)
Pfam:
InterPro:
- Nucleotide-binding alpha-beta plait domain superfamily
- Peptidyl-prolyl cis-trans isomerase E
- RNA-binding domain superfamily
- RNA recognition motif domain
- Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif
- Cyclophilin-like domain superfamily
- Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
- Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site
Chain: 2
Length: 646 amino acids
Theoretical weight: 73.68 KDa
Source organism: Homo sapiens
UniProt:
Pfam:
InterPro:
Length: 646 amino acids
Theoretical weight: 73.68 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q96BP3 (Residues: 1-646; Coverage: 100%)
Pfam:
InterPro:
Chain: 3
Length: 754 amino acids
Theoretical weight: 88.85 KDa
Source organism: Homo sapiens
UniProt:
Pfam: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
InterPro:
Length: 754 amino acids
Theoretical weight: 88.85 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: Q13427 (Residues: 1-754; Coverage: 100%)
Pfam: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
InterPro:
Name:
U5 snRNA
Representative chains: B
Length: 117 nucleotides
Theoretical weight: 37.25 KDa
Rfam: U5 spliceosomal RNA
Representative chains: B
Length: 117 nucleotides
Theoretical weight: 37.25 KDa
Rfam: U5 spliceosomal RNA
Name:
U6 snRNA
Representative chains: F
Length: 107 nucleotides
Theoretical weight: 34.4 KDa
Rfam: U6 spliceosomal RNA
Representative chains: F
Length: 107 nucleotides
Theoretical weight: 34.4 KDa
Rfam: U6 spliceosomal RNA
Name:
U2 snRNA
Representative chains: H
Length: 188 nucleotides
Theoretical weight: 60.19 KDa
Rfam: U2 spliceosomal RNA
Representative chains: H
Length: 188 nucleotides
Theoretical weight: 60.19 KDa
Rfam: U2 spliceosomal RNA