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Bacterial GyrB ATPase domain in complex with (3,4-dichlorophenyl)hydrazine

X-ray diffraction
2.1Å resolution
PDB entry 5z9b coloured by chain, front view.
PDB entry 5z9b coloured by chain, side view.
PDB entry 5z9b coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA gyrase subunit B
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Multimeric state: monomeric
Accessible surface area: 9332.32 Å2
Buried surface area: 273.96 Å2
Dissociation area: 57.93 Å2
Dissociation energy (ΔGdiss): 2.76 kcal/mol
Dissociation entropy (TΔSdiss): 0.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142496
Assembly 2
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Assembly Name: DNA gyrase subunit B
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Multimeric state: monomeric
Accessible surface area: 9243.71 Å2
Buried surface area: 263.91 Å2
Dissociation area: 52.03 Å2
Dissociation energy (ΔGdiss): 2.32 kcal/mol
Dissociation entropy (TΔSdiss): 0.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142496

Macromolecules

DNA gyrase subunit B
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES6 (Residues: 15-221; Coverage: 26%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Pfam: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
InterPro:
CATH: Histidine kinase-like ATPase, C-terminal domain
DNA gyrase subunit B in PDB entry 5z9b, assembly 1, front view.
DNA gyrase subunit B in PDB entry 5z9b, assembly 1, side view.
DNA gyrase subunit B in PDB entry 5z9b, assembly 1, top view.
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