› › Structure analysis

Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate

Electron Microscopy
5.7Å resolution
PDB entry 5zam coloured by chain, front view.
PDB entry 5zam coloured by chain, side view.
PDB entry 5zam coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: RISC-loading complex, TARBP2 variant and RNA
hide full assembly name
Multimeric state: hetero trimer
Accessible surface area: 76174.41 Å2
Buried surface area: 4722.9 Å2
Dissociation area: 1,073.27 Å2
Dissociation energy (ΔGdiss): 4.23 kcal/mol
Dissociation entropy (TΔSdiss): 11.52 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-172350

Macromolecules

Endoribonuclease Dicer
Chain: A
Length: 1922 amino acids
Theoretical weight: 218.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UPY3 (Residues: 1-1922; Coverage: 100%)
Gene names: DICER, DICER1, HERNA, KIAA0928
Pfam:
InterPro:
Endoribonuclease Dicer in PDB entry 5zam, assembly 1, front view.
Endoribonuclease Dicer in PDB entry 5zam, assembly 1, side view.
Endoribonuclease Dicer in PDB entry 5zam, assembly 1, top view.
Molecule details ›

Search similar proteins

RISC-loading complex subunit TARBP2
Chain: B
Length: 366 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15633 (Residues: 1-366; Coverage: 100%)
Gene names: TARBP2, TRBP
Pfam: Double-stranded RNA binding motif
InterPro:
RISC-loading complex subunit TARBP2 in PDB entry 5zam, assembly 1, front view.
RISC-loading complex subunit TARBP2 in PDB entry 5zam, assembly 1, side view.
RISC-loading complex subunit TARBP2 in PDB entry 5zam, assembly 1, top view.
Molecule details ›

Search similar proteins

RNA chain C
Name: RNA (73-mer)
Representative chains: C
Length: 73 nucleotides
Theoretical weight: 23.38 KDa
Rfam: let-7 microRNA precursor
RNA (73-mer) in PDB entry 5zam, assembly 1, front view.
RNA (73-mer) in PDB entry 5zam, assembly 1, side view.
RNA (73-mer) in PDB entry 5zam, assembly 1, top view.
Molecule details ›

Search similar RNA

Quick links