› 6n33

X-ray diffraction
2.25Å resolution

Crystal structure of fms kinase domain with a small molecular inhibitor, PLX5622

Released:
DOI: 10.2210/pdb6n33/pdb
PDB entry 6n33 coloured by chain, front view.
PDB entry 6n33 coloured by chain, side view.
PDB entry 6n33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer's disease model.
Spangenberg E, Severson PL, Hohsfield LA, Crapser J, Zhang J, Burton EA, Zhang Y, Spevak W, Lin J, Phan NY, Habets G, Rymar A, Tsang G, Walters J, Nespi M, Singh P, Broome S, Ibrahim P, Zhang C, Bollag G, West BL, Green KN
OpenAccess logo Nat Commun 10 3758 (2019)
PMID: 31434879

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage colony-stimulating factor 1 receptor Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07333 (Residues: 542-919; Coverage: 35%)
Gene names: CSF1R, FMS
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

1 bound ligand:
Ligand 622 1 x 622
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P43212
Unit cell:
a: 62.62Å b: 62.62Å c: 182.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Spodoptera frugiperda

Quick links

Citations

93 review citations

Alzheimer Disease: An Update on Pathobiology and Treatment Strategies.
Long et al. (2019)
92 more

2 mentions without citation

Engineering an inhibitor-resistant human CSF1R variant for microglia replacement.
Chadarevian et al. (2023)
1 more