› 6trh

X-ray diffraction
1.86Å resolution

Structure of E70A mutant of Rex8A from Paenibacillus barcinonensis complexed with 3(3)-alpha-L-arabinofuranosyl-xylotetraose.

Released:
DOI: 10.2210/pdb6trh/pdb
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PDB entry 6trh coloured by chain, front view.
PDB entry 6trh coloured by chain, side view.
PDB entry 6trh coloured by chain, top view.
Source organism: Paenibacillus barcinonensis
Primary publication:
Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.
Jiménez-Ortega E, Valenzuela S, Ramírez-Escudero M, Pastor FJ, Sanz-Aparicio J
FEBS J 287 5362-5374 (2020)
PMID: 32352213

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Reducing-end xylose-releasing exo-oligoxylanase Rex8A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 385 amino acids
Theoretical weight: 44.68 KDa
Source organism: Paenibacillus barcinonensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S2UQQ5 (Residues: 1-380; Coverage: 100%)
Gene names: DFQ00_11062, rex8A
Sequence domains: Glycosyl hydrolases family 8

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, AHR
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2221
Unit cell:
a: 82.295Å b: 82.72Å c: 465.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Comprehensive Genome Analysis of Cellulose and Xylan-Active CAZymes from the Genus Paenibacillus: Special Emphasis on the Novel Xylanolytic Paenibacillus sp. LS1.
Mukherjee et al. (2023)
1 more

2 mentions without citation

Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii.
Kmezik et al. (2021)
1 more

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