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Structure of E70A mutant of Rex8A from Paenibacillus barcinonensis complexed with 3(3)-alpha-L-arabinofuranosyl-xylotetraose.

X-ray diffraction
1.86Å resolution
PDB entry 6trh coloured by chain, front view.
PDB entry 6trh coloured by chain, side view.
PDB entry 6trh coloured by chain, top view.
Source organism: Paenibacillus barcinonensis
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Reducing-end xylose-releasing exo-oligoxylanase Rex8A
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Multimeric state: monomeric
Accessible surface area: 14307.24 Å2
Buried surface area: 1775.27 Å2
Dissociation area: 646.69 Å2
Dissociation energy (ΔGdiss): -5.26 kcal/mol
Dissociation entropy (TΔSdiss): 6.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102692
Assembly 2
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Assembly Name: Reducing-end xylose-releasing exo-oligoxylanase Rex8A
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Multimeric state: monomeric
Accessible surface area: 14336.35 Å2
Buried surface area: 1764.02 Å2
Dissociation area: 640.85 Å2
Dissociation energy (ΔGdiss): -5.18 kcal/mol
Dissociation entropy (TΔSdiss): 6.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102692
Assembly 3
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Assembly Name: Reducing-end xylose-releasing exo-oligoxylanase Rex8A
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Multimeric state: monomeric
Accessible surface area: 14642.06 Å2
Buried surface area: 1768.03 Å2
Dissociation area: 642.78 Å2
Dissociation energy (ΔGdiss): -4.36 kcal/mol
Dissociation entropy (TΔSdiss): 6.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102692
Assembly 4
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Assembly Name: Reducing-end xylose-releasing exo-oligoxylanase Rex8A
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Multimeric state: monomeric
Accessible surface area: 14132.15 Å2
Buried surface area: 1767.64 Å2
Dissociation area: 642.43 Å2
Dissociation energy (ΔGdiss): -5.26 kcal/mol
Dissociation entropy (TΔSdiss): 6.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102692

Macromolecules

Reducing-end xylose-releasing exo-oligoxylanase Rex8A
Chains: A, B, C, D
Length: 385 amino acids
Theoretical weight: 44.68 KDa
Source organism: Paenibacillus barcinonensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S2UQQ5 (Residues: 1-380; Coverage: 100%)
Gene names: DFQ00_11062, rex8A
Pfam: Glycosyl hydrolases family 8
InterPro:
Reducing-end xylose-releasing exo-oligoxylanase Rex8A in PDB entry 6trh, assembly 1, front view.
Reducing-end xylose-releasing exo-oligoxylanase Rex8A in PDB entry 6trh, assembly 1, side view.
Reducing-end xylose-releasing exo-oligoxylanase Rex8A in PDB entry 6trh, assembly 1, top view.
PDBe-KB: UniProt Coverage View: A0A0S2UQQ5  
138550100150200250300350
 
100200300
UniProt
A0A0S2UQQ5
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