› 6xmj

Electron Microscopy
3Å resolution

Human 20S proteasome bound to an engineered 11S (PA26) activator

Released:
DOI: 10.2210/pdb6xmj/pdb
PDB entry 6xmj coloured by chain, front view.
PDB entry 6xmj coloured by chain, side view.
PDB entry 6xmj coloured by chain, top view.
Source organisms:
Primary publication:
The YΦ motif defines the structure-activity relationships of human 20S proteasome activators.
Opoku-Nsiah KA, de la Pena AH, Williams SK, Chopra N, Sali A, Lander GC, Gestwicki JE
OpenAccess logo Nat Commun 13 1226 (2022)
PMID: 35264557
Related structures: EMD-22259

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-518195 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Proteasome subunit alpha type-6 Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 2-245; Coverage: 99%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chain: B
Molecule details ›
Chain: B
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 2-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: C
Molecule details ›
Chain: C
Length: 250 amino acids
Theoretical weight: 28.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 2-251; Coverage: 96%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 27.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 2-244; Coverage: 98%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chain: E
Molecule details ›
Chain: E
Length: 234 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 8-241; Coverage: 97%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: F
Molecule details ›
Chain: F
Length: 238 amino acids
Theoretical weight: 26.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 4-241; Coverage: 91%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chain: G
Molecule details ›
Chain: G
Length: 245 amino acids
Theoretical weight: 27.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 2-246; Coverage: 96%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit beta type-6 Chain: H
Molecule details ›
Chain: H
Length: 202 amino acids
Theoretical weight: 21.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 35-236; Coverage: 85%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chain: I
Molecule details ›
Chain: I
Length: 220 amino acids
Theoretical weight: 23.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 44-263; Coverage: 79%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chain: J
Molecule details ›
Chain: J
Length: 204 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 2-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chain: K
Molecule details ›
Chain: K
Length: 199 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-199; Coverage: 99%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chain: L
Molecule details ›
Chain: L
Length: 201 amino acids
Theoretical weight: 22.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 60-260; Coverage: 76%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chain: M
Molecule details ›
Chain: M
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 29-241; Coverage: 88%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chain: N
Molecule details ›
Chain: N
Length: 217 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 46-262; Coverage: 82%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome activator PA28 C-terminal domain-containing protein Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 228 amino acids
Theoretical weight: 24.96 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9U8G2 (Residues: 4-226; Coverage: 97%)
Sequence domains: Proteasome activator PA28, C-terminal

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-22259
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21

Quick links

Citations

6 citation in other articles

Cryo-EM structure of the plant 26S proteasome.
Kandolf et al. (2022)
5 more

1 mention without citation

Proteasome Biology: Chemistry and Bioengineering Insights.
Račková et al. (2020)