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Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36E at cryogenic temperature

X-ray diffraction
1.85Å resolution
PDB entry 6amf coloured by chain, front view.
PDB entry 6amf coloured by chain, side view.
PDB entry 6amf coloured by chain, top view.
Source organism: Staphylococcus aureus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclease A
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Multimeric state: monomeric
Accessible surface area: 6879.32 Å2
Buried surface area: 831.57 Å2
Dissociation area: 373.21 Å2
Dissociation energy (ΔGdiss): 3.56 kcal/mol
Dissociation entropy (TΔSdiss): 4.77 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132790

Macromolecules

Nuclease A
Chain: A
Length: 143 amino acids
Theoretical weight: 16.19 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Pfam: Staphylococcal nuclease homologue
InterPro:
CATH: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Nuclease A in PDB entry 6amf, assembly 1, front view.
Nuclease A in PDB entry 6amf, assembly 1, side view.
Nuclease A in PDB entry 6amf, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P00644  
114320406080100120140
 
50100
UniProt
P00644
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Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Sequence conservation
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