› 6b0e

X-ray diffraction
3.3Å resolution

Crystal structure of Pfs25 in complex with the transmission blocking antibody 1260

Released:
DOI: 10.2210/pdb6b0e/pdb
PDB entry 6b0e coloured by chain, front view.
PDB entry 6b0e coloured by chain, side view.
PDB entry 6b0e coloured by chain, top view.
Source organisms:
Primary publication:
Molecular definition of multiple sites of antibody inhibition of malaria transmission-blocking vaccine antigen Pfs25.
Scally SW, McLeod B, Bosch A, Miura K, Liang Q, Carroll S, Reponen S, Nguyen N, Giladi E, Rämisch S, Yusibov V, Bradley A, Lemiale F, Schief WR, Emerling D, Kellam P, King CR, Julien JP
OpenAccess logo Nat Commun 8 1568 (2017)
PMID: 29146922

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230861 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
1260 antibody, light chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
1260 antibody, heavy chain Chain: B
Molecule details ›
Chain: B
Length: 227 amino acids
Theoretical weight: 24.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
25 kDa ookinete surface antigen Chain: E
Molecule details ›
Chain: E
Length: 183 amino acids
Theoretical weight: 20.1 KDa
Source organism: Plasmodium falciparum NF54
Expression system: Homo sapiens
UniProt:
  • Canonical: P13829 (Residues: 22-193; Coverage: 86%)
Sequence domains: Pvs28 EGF domain
Structure domains: Plasmodium vivax P25 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 53.223Å b: 79.635Å c: 78.065Å
α: 90° β: 90.91° γ: 90°
R-values:
R R work R free
0.233 0.23 0.28
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Malaria Vaccines: Recent Advances and New Horizons.
Draper et al. (2018)
6 more

2 mentions without citation

Molecular definition of multiple sites of antibody inhibition of malaria transmission-blocking vaccine antigen Pfs25.
Scally et al. (2017)
1 more