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Mechanisms of opening and closing of the bacterial replicative helicase.

<div class='caption-title'>Initiation of DNA replication in bacteria and the assembly pathway for the replicative helicase.</div>
<div class='caption-title'>Architecture and stoichiometry of the E.coli DnaB helicase•bacteriophage λP complex.</div>
<div class='caption-title'>Architecture and biochemistry of the E.coli DnaB helicase and the bacteriophage λP helicase loader.</div>
Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D
OpenAccess logo Elife 7 (2018)
Cited: 12 times
EuropePMC logo PMID: 30582519

Abstract

Assembly of bacterial ring-shaped hexameric replicative helicases on single-stranded (ss) DNA requires specialized loading factors. However, mechanisms implemented by these factors during opening and closing of the helicase, which enable and restrict access to an internal chamber, are not known. Here, we investigate these mechanisms in the Escherichia coli DnaB helicase•bacteriophage λ helicase loader (λP) complex. We show that five copies of λP bind at DnaB subunit interfaces and reconfigure the helicase into an open spiral conformation that is intermediate to previously observed closed ring and closed spiral forms; reconfiguration also produces openings large enough to admit ssDNA into the inner chamber. The helicase is also observed in a restrained inactive configuration that poises it to close on activating signal, and transition to the translocation state. Our findings provide insights into helicase opening, delivery to the origin and ssDNA entry, and closing in preparation for translocation.

Articles - 6bbm mentioned but not cited (4)

  1. Mechanisms of opening and closing of the bacterial replicative helicase. Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D. Elife 7 e41140 (2018)
  2. Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage. Hamidian M, Blasco L, Tillman LN, To J, Tomas M, Myers GSA. Microorganisms 8 E1851 (2020)
  3. The Caulobacter crescentus DciA promotes chromosome replication through topological loading of the DnaB replicative helicase at replication forks. Ozaki S, Wang D, Wakasugi Y, Itani N, Katayama T. Nucleic Acids Res 50 12896-12912 (2022)
  4. The LH-DH module of bacterial replicative helicases is the common binding site for DciA and other helicase loaders. Cargemel C, Marsin S, Noiray M, Legrand P, Bounoua H, Li de la Sierra-Gallay I, Walbott H, Quevillon-Cheruel S. Acta Crystallogr D Struct Biol 79 177-187 (2023)


Reviews citing this publication (3)

  1. A structural view of bacterial DNA replication. Oakley AJ. Protein Sci 28 990-1004 (2019)
  2. Mechanisms of hexameric helicases. Fernandez AJ, Berger JM. Crit Rev Biochem Mol Biol 56 621-639 (2021)
  3. Convergent evolution in two bacterial replicative helicase loaders. Chase J, Berger J, Jeruzalmi D. Trends Biochem Sci 47 620-630 (2022)

Articles citing this publication (5)

  1. DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation. Adam S, Anteneh H, Hornisch M, Wagner V, Lu J, Radde NE, Bashtrykov P, Song J, Jeltsch A. Nat Commun 11 3723 (2020)
  2. Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM. Olinares PDB, Kang JY, Llewellyn E, Chiu C, Chen J, Malone B, Saecker RM, Campbell EA, Darst SA, Chait BT. Structure 29 186-195.e6 (2021)
  3. Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Marsin S, Adam Y, Cargemel C, Andreani J, Baconnais S, Legrand P, Li de la Sierra-Gallay I, Humbert A, Aumont-Nicaise M, Velours C, Ochsenbein F, Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL. Nucleic Acids Res 49 6569-6586 (2021)
  4. Deciphering the mechanism and function of Hsp100 unfoldases from protein structure. Lee G, Kim RS, Lee SB, Lee S, Tsai FTF. Biochem Soc Trans 50 1725-1736 (2022)
  5. Structural basis of the T4 bacteriophage primosome assembly and primer synthesis. Feng X, Spiering MM, de Luna Almeida Santos R, Benkovic SJ, Li H. Nat Commun 14 4396 (2023)


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