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Crystal structure of a membrane protein, crystal form II

X-ray diffraction
3.15Å resolution
PDB entry 6buh coloured by chain, front view.
PDB entry 6buh coloured by chain, side view.
PDB entry 6buh coloured by chain, top view.
Source organism: Streptococcus thermophilus
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Teichoic acid D-alanyltransferase and D-alanyl carrier protein
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Multimeric state: hetero dimer
Accessible surface area: 25585.2 Å2
Buried surface area: 1491.93 Å2
Dissociation area: 745.96 Å2
Dissociation energy (ΔGdiss): 2.01 kcal/mol
Dissociation entropy (TΔSdiss): 11.44 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-177593
Assembly 2
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Assembly Name: Teichoic acid D-alanyltransferase and D-alanyl carrier protein
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Multimeric state: hetero dimer
Accessible surface area: 25311.35 Å2
Buried surface area: 1496.63 Å2
Dissociation area: 748.32 Å2
Dissociation energy (ΔGdiss): 2.17 kcal/mol
Dissociation entropy (TΔSdiss): 11.43 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-177593
Assembly 3
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Assembly Name: Teichoic acid D-alanyltransferase and D-alanyl carrier protein
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Multimeric state: hetero dimer
Accessible surface area: 25447.84 Å2
Buried surface area: 1512.89 Å2
Dissociation area: 756.45 Å2
Dissociation energy (ΔGdiss): 3.18 kcal/mol
Dissociation entropy (TΔSdiss): 11.44 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-177593
Assembly 4
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Assembly Name: Teichoic acid D-alanyltransferase and D-alanyl carrier protein
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Multimeric state: hetero dimer
Accessible surface area: 25475.38 Å2
Buried surface area: 1528.15 Å2
Dissociation area: 764.07 Å2
Dissociation energy (ΔGdiss): 0.43 kcal/mol
Dissociation entropy (TΔSdiss): 11.44 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-177593

Macromolecules

D-alanyl carrier protein
Chains: A, B, E, G
Length: 82 amino acids
Theoretical weight: 9.34 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M4V3 (Residues: 1-79; Coverage: 100%)
Gene names: dltC, stu0763
Pfam: Phosphopantetheine attachment site
InterPro:
CATH: ACP-like
D-alanyl carrier protein in PDB entry 6buh, assembly 1, front view.
D-alanyl carrier protein in PDB entry 6buh, assembly 1, side view.
D-alanyl carrier protein in PDB entry 6buh, assembly 1, top view.
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Teichoic acid D-alanyltransferase
Chains: C, D, F, H
Length: 425 amino acids
Theoretical weight: 50.19 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M4V4 (Residues: 1-415; Coverage: 100%)
Gene names: dltB, stu0762
Pfam: MBOAT, membrane-bound O-acyltransferase family
InterPro:
Teichoic acid D-alanyltransferase in PDB entry 6buh, assembly 1, front view.
Teichoic acid D-alanyltransferase in PDB entry 6buh, assembly 1, side view.
Teichoic acid D-alanyltransferase in PDB entry 6buh, assembly 1, top view.
Molecule details ›

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