6dnc

Electron Microscopy
3.7Å resolution

E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon

Released:
Source organism: Escherichia coli
Primary publication:
Mechanism of premature translation termination on a sense codon.
J Biol Chem 293 12472-12479 (2018)
PMID: 29941456
Related structures: EMD-7970

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 59-mer (preferred)
PDBe Complex ID:
PDB-CPX-249800 (preferred)
Entry contents:
53 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (58 distinct):
Large ribosomal subunit protein uL1 Chain: E
Molecule details ›
Chain: E
Length: 234 amino acids
Theoretical weight: 24.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 1-234; Coverage: 100%)
Gene names: JW3947, b3984, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL2 Chain: F
Molecule details ›
Chain: F
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: G
Large ribosomal subunit protein uL4 Chain: H
Large ribosomal subunit protein uL5 Chain: I
Large ribosomal subunit protein uL6 Chain: J
Large ribosomal subunit protein bL9 Chain: K
Large ribosomal subunit protein uL10 Chain: L
Large ribosomal subunit protein uL11 Chain: M
Large ribosomal subunit protein uL13 Chain: N
Large ribosomal subunit protein uL14 Chain: O
Large ribosomal subunit protein uL15 Chain: P
Large ribosomal subunit protein uL16 Chain: Q
Large ribosomal subunit protein bL17 Chain: R
Large ribosomal subunit protein uL18 Chain: S
Large ribosomal subunit protein bL19 Chain: T
Large ribosomal subunit protein bL20 Chain: U
Large ribosomal subunit protein bL21 Chain: V
Large ribosomal subunit protein uL22 Chain: W
Large ribosomal subunit protein uL23 Chain: X
Large ribosomal subunit protein uL24 Chain: Y
Large ribosomal subunit protein bL25 Chain: Z
Large ribosomal subunit protein bL27 Chain: AA
Large ribosomal subunit protein bL28 Chain: BA
Large ribosomal subunit protein uL29 Chain: CA
Large ribosomal subunit protein uL30 Chain: DA
Large ribosomal subunit protein bL31 Chain: EA
Large ribosomal subunit protein bL32 Chain: FA
Large ribosomal subunit protein bL33 Chain: GA
Large ribosomal subunit protein bL34 Chain: HA
Large ribosomal subunit protein bL35 Chain: IA
Large ribosomal subunit protein bL36A Chain: JA
Peptide chain release factor RF1 Chain: MA
Small ribosomal subunit protein uS2 Chain: OA
Small ribosomal subunit protein uS3 Chain: PA
Small ribosomal subunit protein uS4 Chain: QA
Small ribosomal subunit protein uS5 Chain: RA
Small ribosomal subunit protein bS6 Chain: SA
Small ribosomal subunit protein uS7 Chain: TA
Small ribosomal subunit protein uS8 Chain: UA
Small ribosomal subunit protein uS9 Chain: VA
Small ribosomal subunit protein uS10 Chain: WA
Small ribosomal subunit protein uS11 Chain: XA
Small ribosomal subunit protein uS12 Chain: YA
Small ribosomal subunit protein uS13 Chain: ZA
Small ribosomal subunit protein uS14 Chain: AB
Small ribosomal subunit protein uS15 Chain: BB
Small ribosomal subunit protein bS16 Chain: CB
Small ribosomal subunit protein uS17 Chain: DB
Small ribosomal subunit protein bS18 Chain: EB
Small ribosomal subunit protein uS19 Chain: FB
Small ribosomal subunit protein bS20 Chain: GB
Small ribosomal subunit protein bS21 Chain: HB
16S ribosomal RNA Chain: A
23S ribosomal RNA Chain: B
5S ribosomal RNA Chain: C
tRNA(fMet) Chains: D, LA
Messenger RNA Chain: KA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-7970
Expression system: Escherichia coli