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Bst DNA polymerase I post-chemistry (n+2) structure

X-ray diffraction
1.99Å resolution
PDB entry 6dsv coloured by chain, front view.
PDB entry 6dsv coloured by chain, side view.
PDB entry 6dsv coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase I and DNA
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Multimeric state: hetero trimer
Accessible surface area: 27657.64 Å2
Buried surface area: 4366.82 Å2
Dissociation area: 1,267.56 Å2
Dissociation energy (ΔGdiss): 19.76 kcal/mol
Dissociation entropy (TΔSdiss): 10.45 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119803

Macromolecules

DNA polymerase I
Chain: A
Length: 580 amino acids
Theoretical weight: 66.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1C9K5 (Residues: 1-580; Coverage: 100%)
Gene name: polA
Pfam:
InterPro:
CATH:
DNA polymerase I in PDB entry 6dsv, assembly 1, front view.
DNA polymerase I in PDB entry 6dsv, assembly 1, side view.
DNA polymerase I in PDB entry 6dsv, assembly 1, top view.
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DNA chain P
Name: DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3')
Representative chains: P
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3') in PDB entry 6dsv, assembly 1, front view.
DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3') in PDB entry 6dsv, assembly 1, side view.
DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3') in PDB entry 6dsv, assembly 1, top view.
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DNA chain T
Name: DNA (5'-D(P*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3')
Representative chains: T
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
DNA (5'-D(P*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3') in PDB entry 6dsv, assembly 1, front view.
DNA (5'-D(P*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3') in PDB entry 6dsv, assembly 1, side view.
DNA (5'-D(P*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3') in PDB entry 6dsv, assembly 1, top view.
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