› 6efx

X-ray diffraction
2Å resolution

Crystal structure of a YjeF family protein from Cryptococcus neoformans var. grubii serotype A in complex with AMPPNP

Released:
DOI: 10.2210/pdb6efx/pdb
PDB entry 6efx coloured by chain, front view.
PDB entry 6efx coloured by chain, side view.
PDB entry 6efx coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-125417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent (S)-NAD(P)H-hydrate dehydratase Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 38.3 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: J9VIT7 (Residues: 1-346; Coverage: 100%)
Gene name: CNAG_05097
Sequence domains: Carbohydrate kinase

Ligands and Environments

4 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: I422
Unit cell:
a: 85.34Å b: 85.34Å c: 187.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.164 0.211
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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