› 6eq0

X-ray diffraction
2.45Å resolution

Structure of the periplasmic binding protein (PBP) MelB (atu4661) in complex with galactose from agrobacterium tumefacien C58

Released:
DOI: 10.2210/pdb6eq0/pdb
PDB entry 6eq0 coloured by chain, front view.
PDB entry 6eq0 coloured by chain, side view.
PDB entry 6eq0 coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
The plant defense signal galactinol is specifically used as a nutrient by the bacterial pathogen Agrobacterium fabrum.
Meyer T, Vigouroux A, Aumont-Nicaise M, Comte G, Vial L, Lavire C, Moréra S
J Biol Chem 293 7930-7941 (2018)
PMID: 29602905

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic alpha-galactoside-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 683 amino acids
Theoretical weight: 76.36 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli

Ligands and Environments

5 bound ligands:
Ligand GLA 2 x GLA
Ligand PEG 2 x PEG
Ligand EDO 21 x EDO
Ligand CL 16 x CL
Ligand CA 17 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 107.84Å b: 73.92Å c: 171.1Å
α: 90° β: 92.55° γ: 90°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors.
Meyer et al. (2019)
9 other articles

2 mentions without citation

The plant defense signal galactinol is specifically used as a nutrient by the bacterial pathogen Agrobacterium fabrum.
Meyer et al. (2018)
1 more