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The plant defense signal galactinol is specifically used as a nutrient by the bacterial pathogen Agrobacterium fabrum.

Meyer T, Vigouroux A, Aumont-Nicaise M, Comte G, Vial L, Lavire C, Moréra S
J Biol Chem 293 7930-7941 (2018)
Related entries: 6epy, 6epz, 6eq1, 6eq8

Cited: 16 times
EuropePMC logo PMID: 29602905

Abstract

The bacterial plant pathogen Agrobacterium fabrum uses periplasmic-binding proteins (PBPs) along with ABC transporters to import a wide variety of plant molecules as nutrients. Nonetheless, how A. fabrum acquires plant metabolites is incompletely understood. Using genetic approaches and affinity measurements, we identified here the PBP MelB and its transporter as being responsible for the uptake of the raffinose family of oligosaccharides (RFO), which are the most widespread d-galactose-containing oligosaccharides in higher plants. We also found that the RFO precursor galactinol, recently described as a plant defense molecule, is imported into Agrobacterium via MelB with nanomolar range affinity. Structural analyses and binding mode comparisons of the X-ray structures of MelB in complex with raffinose, stachyose, galactinol, galactose, and melibiose (a raffinose degradation product) revealed how MelB recognizes the nonreducing end galactose common to all these ligands and that MelB has a strong preference for a two-unit sugar ligand. Of note, MelB conferred a competitive advantage to A. fabrum in colonizing the rhizosphere of tomato plants. Our integrative work highlights the structural and functional characteristics of melibiose and galactinol assimilation by A. fabrum, leading to a competitive advantage for these bacteria in the rhizosphere. We propose that the PBP MelB, which is highly conserved among both symbionts and pathogens from Rhizobiace family, is a major trait in these bacteria required for early steps of plant colonization.

Articles - 6eq0 mentioned but not cited (3)

  1. The plant defense signal galactinol is specifically used as a nutrient by the bacterial pathogen Agrobacterium fabrum. Meyer T, Vigouroux A, Aumont-Nicaise M, Comte G, Vial L, Lavire C, Moréra S. J Biol Chem 293 7930-7941 (2018)
  2. Benchmark Sets for Binding Hot Spot Identification in Fragment-Based Ligand Discovery. Wakefield AE, Yueh C, Beglov D, Castilho MS, Kozakov D, Keserű GM, Whitty A, Vajda S. J Chem Inf Model 60 6612-6623 (2020)
  3. Conservation of Hot Spots and Ligand Binding Sites in Protein Models by AlphaFold2. Bekar-Cesaretli AA, Khan O, Nguyen T, Kozakov D, Joseph-Mccarthy D, Vajda S. J Chem Inf Model 64 960-973 (2024)


Reviews citing this publication (1)

  1. Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors. Meyer T, Thiour-Mauprivez C, Wisniewski-Dyé F, Kerzaon I, Comte G, Vial L, Lavire C. Front Plant Sci 10 978 (2019)

Articles citing this publication (12)

  1. Secondary metabolites from plant-associated Pseudomonas are overproduced in biofilm. Rieusset L, Rey M, Muller D, Vacheron J, Gerin F, Dubost A, Comte G, Prigent-Combaret C. Microb Biotechnol 13 1562-1580 (2020)
  2. Galactinol synthase 1 improves cucumber performance under cold stress by enhancing assimilate translocation. Dai H, Zhu Z, Wang Z, Zhang Z, Kong W, Miao M. Hortic Res uhab063 (2022)
  3. Root exudate-derived compounds stimulate the phosphorus solubilizing ability of bacteria. Pantigoso HA, Manter DK, Fonte SJ, Vivanco JM. Sci Rep 13 4050 (2023)
  4. Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice. Mukherjee S, Sengupta S, Mukherjee A, Basak P, Majumder AL. Planta 249 891-912 (2019)
  5. Multiple Metabolic Phenotypes as Screening Criteria Are Correlated With the Plant Growth-Promoting Ability of Rhizobacterial Isolates. Shi P, Zhang J, Li X, Zhou L, Luo H, Wang L, Zhang Y, Chou M, Wei G. Front Microbiol 12 747982 (2021)
  6. Reconstruction and analysis of a genome-scale metabolic model for Agrobacterium tumefaciens. Xu N, Yang Q, Yang X, Wang M, Guo M. Mol Plant Pathol 22 348-360 (2021)
  7. Pan-Chromosome and Comparative Analysis of Agrobacterium fabrum Reveal Important Traits Concerning the Genetic Diversity, Evolutionary Dynamics, and Niche Adaptation of the Species. Du Y, Zou J, Yin Z, Chen T. Microbiol Spectr e0292422 (2023)
  8. Isolation and identification of a new Bacillus glycinifermentans strain from date palm rhizosphere and its effect on barley seeds under heavy metal stress. Belhassan M, Farhat A, Abed HE, Chaabeen Z, Bouzid F, Elleuch A, Fendri I, Khemakhem B. Braz J Microbiol 55 843-854 (2024)
  9. Solanum lycopersicum Seedlings. Metabolic Responses Induced by the Alkamide Affinin. Campos-García T, Molina-Torres J. Metabolites 11 143 (2021)
  10. Mulberry MnGolS2 Mediates Resistance to Botrytis cinerea on Transgenic Plants. Wang D, Liu Z, Qin Y, Zhang S, Yang L, Shang Q, Ji X, Xin Y, Li X. Genes (Basel) 14 1912 (2023)
  11. Root-associated bacterial communities and root metabolite composition are linked to nitrogen use efficiency in sorghum. Chai YN, Qi Y, Goren E, Chiniquy D, Sheflin AM, Tringe SG, Prenni JE, Liu P, Schachtman DP. mSystems 9 e0119023 (2024)
  12. The timing of bacterial mesophyll infection shapes the leaf chemical landscape. Roman-Reyna V, Heiden N, Butchacas J, Toth H, Cooperstone JL, Jacobs JM. Microbiol Spectr 12 e0413823 (2024)


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