› 6euy

X-ray diffraction
3Å resolution

Structure of the midlink and cap-binding domains of influenza A polymerase PB2 subunit with a bound azaindazole cap-binding inhibitor

Released:
DOI: 10.2210/pdb6euy/pdb
PDB entry 6euy coloured by chain, front view.
PDB entry 6euy coloured by chain, side view.
PDB entry 6euy coloured by chain, top view.
Source organism: Influenza A virus (A/Victoria/3/1975(H3N2))
Primary publication:
Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Pflug A, Gaudon S, Resa-Infante P, Lethier M, Reich S, Schulze WM, Cusack S
OpenAccess logo Nucleic Acids Res 46 956-971 (2018)
PMID: 29202182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 290 amino acids
Theoretical weight: 32.52 KDa
Source organism: Influenza A virus (A/Victoria/3/1975(H3N2))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31345 (Residues: 247-536; Coverage: 38%)
Gene name: PB2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BYB 3 x BYB
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P2221
Unit cell:
a: 49.461Å b: 112.322Å c: 201.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structure and Function of the Influenza Virus Transcription and Replication Machinery.
Fodor et al. (2020)
5 more

3 mentions without citation

Revisiting Viral RNA-Dependent RNA Polymerases: Insights from Recent Structural Studies.
Ramaswamy et al. (2022)
2 more