› 6f4h

X-ray diffraction
2Å resolution

Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex

Released:
DOI: 10.2210/pdb6f4h/pdb
PDB entry 6f4h coloured by chain, front view.
PDB entry 6f4h coloured by chain, side view.
PDB entry 6f4h coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.
Weber G, DeKoster GT, Holton N, Hall KB, Wahl MC
OpenAccess logo Nat Commun 9 2220 (2018)
PMID: 29880797

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-545410 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 98 amino acids
Theoretical weight: 11.08 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P43332 (Residues: 1-96; Coverage: 44%)
Gene names: CG4528, D25, fs(1)1621, liz, snf
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
U1-SL2 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 22 nucleotides
Theoretical weight: 7 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 56.705Å b: 150.028Å c: 133.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.
van der Feltz et al. (2019)
3 other articles

2 mentions without citation

Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.
Weber et al. (2018)
1 more