6f4h › Citations

Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.

Fig. 1
Fig. 2
Fig. 3
Weber G, DeKoster GT, Holton N, Hall KB, Wahl MC
OpenAccess logo Nat Commun 9 2220 (2018)
Related entries: 6f4g, 6f4i, 6f4j

Cited: 6 times
EuropePMC logo PMID: 29880797

Abstract

The first RNA recognition motif of the Drosophila SNF protein is an example of an RNA binding protein with multi-specificity. It binds different RNA hairpin loops in spliceosomal U1 or U2 small nuclear RNAs, and only in the latter case requires the auxiliary U2A' protein. Here we investigate its functions by crystal structures of SNF alone and bound to U1 stem-loop II, U2A' or U2 stem-loop IV and U2A', SNF dynamics from NMR spectroscopy, and structure-guided mutagenesis in binding studies. We find that different loop-closing base pairs and a nucleotide exchange at the tips of the loops contribute to differential SNF affinity for the RNAs. U2A' immobilizes SNF and RNA residues to restore U2 stem-loop IV binding affinity, while U1 stem-loop II binding does not require such adjustments. Our findings show how U2A' can modulate RNA specificity of SNF without changing SNF conformation or relying on direct RNA contacts.

Articles - 6f4h mentioned but not cited (2)

  1. Molecular principles underlying dual RNA specificity in the Drosophila SNF protein. Weber G, DeKoster GT, Holton N, Hall KB, Wahl MC. Nat Commun 9 2220 (2018)
  2. RNA-NRD: a non-redundant RNA structural dataset for benchmarking and functional analysis. Khan NS, Rahaman MM, Islam S, Zhang S. NAR Genom Bioinform 5 lqad040 (2023)


Reviews citing this publication (1)

  1. Structural and functional modularity of the U2 snRNP in pre-mRNA splicing. van der Feltz C, Hoskins AA. Crit Rev Biochem Mol Biol 54 443-465 (2019)

Articles citing this publication (3)

  1. Integrating Phylogenetics With Intron Positions Illuminates the Origin of the Complex Spliceosome. Vosseberg J, Stolker D, von der Dunk SHA, Snel B. Mol Biol Evol 40 msad011 (2023)
  2. Crystal structure of the RNA-recognition motif of Drosophila melanogaster tRNA (uracil-5-)-methyltransferase homolog A. Witzenberger M, Janowski R, Niessing D. Acta Crystallogr F Struct Biol Commun 80 36-42 (2024)
  3. Phylogeny and conservation of plant U2A/U2A', a core splicing component in U2 spliceosomal complex. Liu Y, Tian Y, Wang LX, Fan T, Zhang J, Chen MX, Liu YG. Planta 255 25 (2021)


Quick links