6hjn › Citations

Influenza hemagglutinin membrane anchor.

Fig. 1.
Fig. 2.
Fig. 3.
Benton DJ, Nans A, Calder LJ, Turner J, Neu U, Lin YP, Ketelaars E, Kallewaard NL, Corti D, Lanzavecchia A, Gamblin SJ, Rosenthal PB, Skehel JJ
OpenAccess logo Proc Natl Acad Sci U S A 115 10112-10117 (2018)
Related entries: 6hjp, 6hjq, 6hjr, 6hkg

Cited: 56 times
EuropePMC logo PMID: 30224494

Abstract

Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the "membrane anchor" portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA-detergent micelles with full-length HA-Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high-resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor.

Articles - 6hjn mentioned but not cited (1)

  1. Influenza hemagglutinin membrane anchor. Benton DJ, Nans A, Calder LJ, Turner J, Neu U, Lin YP, Ketelaars E, Kallewaard NL, Corti D, Lanzavecchia A, Gamblin SJ, Rosenthal PB, Skehel JJ. Proc Natl Acad Sci U S A 115 10112-10117 (2018)


Reviews citing this publication (14)

  1. Influenza Hemagglutinin Structures and Antibody Recognition. Wu NC, Wilson IA. Cold Spring Harb Perspect Med 10 a038778 (2020)
  2. Hemagglutinin Structure and Activities. Gamblin SJ, Vachieri SG, Xiong X, Zhang J, Martin SR, Skehel JJ. Cold Spring Harb Perspect Med 11 a038638 (2021)
  3. Effectiveness of supplementation with quercetin-type flavonols for treatment of viral lower respiratory tract infections: Systematic review and meta-analysis of preclinical studies. Brito JCM, Lima WG, Cordeiro LPB, da Cruz Nizer WS. Phytother Res 35 4930-4942 (2021)
  4. Structural Biology of Influenza Hemagglutinin: An Amaranthine Adventure. Wu NC, Wilson IA. Viruses 12 E1053 (2020)
  5. B Cell Responses against Influenza Viruses: Short-Lived Humoral Immunity against a Life-Long Threat. Guthmiller JJ, Utset HA, Wilson PC. Viruses 13 965 (2021)
  6. SARS-CoV-2 and the possible connection to ERs, ACE2, and RAGE: Focus on susceptibility factors. Stilhano RS, Costa AJ, Nishino MS, Shams S, Bartolomeo CS, Breithaupt-Faloppa AC, Silva EA, Ramirez AL, Prado CM, Ureshino RP. FASEB J 34 14103-14119 (2020)
  7. New Biophysical Approaches Reveal the Dynamics and Mechanics of Type I Viral Fusion Machinery and Their Interplay with Membranes. Benhaim MA, Lee KK. Viruses 12 (2020)
  8. COVID-19: Myths and Reality. Kordyukova LV, Shanko AV. Biochemistry (Mosc) 86 800-817 (2021)
  9. Developing Graphene Grids for Cryoelectron Microscopy. Fan H, Sun F. Front Mol Biosci 9 937253 (2022)
  10. Ferritin-based nanomedicine for disease treatment. Zhu Y, Zhu Y, Cao T, Liu X, Liu X, Yan Y, Shi Y, Wang JC. Med Rev (Berl) 3 49-74 (2023)
  11. Inconspicuous Yet Indispensable: The Coronavirus Spike Transmembrane Domain. Aliper ET, Efremov RG. Int J Mol Sci 24 16421 (2023)
  12. Molecular Modeling of Viral Type I Fusion Proteins: Inhibitors of Influenza Virus Hemagglutinin and the Spike Protein of Coronavirus. Borisevich SS, Zarubaev VV, Shcherbakov DN, Yarovaya OI, Salakhutdinov NF. Viruses 15 902 (2023)
  13. Structural Insights for Anti-Influenza Vaccine Design. Han L, Chen C, Han X, Lin S, Ao X, Han X, Wang J, Ye H. Comput Struct Biotechnol J 17 475-483 (2019)
  14. Viral Membrane Fusion and the Transmembrane Domain. Barrett CT, Dutch RE. Viruses 12 (2020)

Articles citing this publication (41)

  1. In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges. Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, van Zandbergen G, Landry J, de Azevedo NTD, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M. Science 370 203-208 (2020)
  2. Biochemical Analysis of Coronavirus Spike Glycoprotein Conformational Intermediates during Membrane Fusion. Kawase M, Kataoka M, Shirato K, Matsuyama S. J Virol 93 (2019)
  3. Cryo-EM Structure of Full-length HIV-1 Env Bound With the Fab of Antibody PG16. Pan J, Peng H, Chen B, Harrison SC. J Mol Biol 432 1158-1168 (2020)
  4. Structural transitions in influenza haemagglutinin at membrane fusion pH. Benton DJ, Gamblin SJ, Rosenthal PB, Skehel JJ. Nature 583 150-153 (2020)
  5. In situ structure and organization of the influenza C virus surface glycoprotein. Halldorsson S, Sader K, Turner J, Calder LJ, Rosenthal PB. Nat Commun 12 1694 (2021)
  6. Broadly neutralizing antibodies target a haemagglutinin anchor epitope. Guthmiller JJ, Han J, Utset HA, Li L, Lan LY, Henry C, Stamper CT, McMahon M, O'Dell G, Fernández-Quintero ML, Freyn AW, Amanat F, Stovicek O, Gentles L, Richey ST, de la Peña AT, Rosado V, Dugan HL, Zheng NY, Tepora ME, Bitar DJ, Changrob S, Strohmeier S, Huang M, García-Sastre A, Liedl KR, Bloom JD, Nachbagauer R, Palese P, Krammer F, Coughlan L, Ward AB, Wilson PC. Nature 602 314-320 (2022)
  7. Designing a chimeric subunit vaccine for influenza virus, based on HA2, M2e and CTxB: a bioinformatics study. Jafari D, Malih S, Gomari MM, Safari M, Jafari R, Farajollahi MM. BMC Mol Cell Biol 21 89 (2020)
  8. Hydrogen-Deuterium Exchange Supports Independent Membrane-Interfacial Fusion Peptide and Transmembrane Domains in Subunit 2 of Influenza Virus Hemagglutinin Protein, a Structured and Aqueous-Protected Connection between the Fusion Peptide and Soluble Ectodomain, and the Importance of Membrane Apposition by the Trimer-of-Hairpins Structure. Ranaweera A, Ratnayake PU, Ekanayaka EAP, Declercq R, Weliky DP. Biochemistry 58 2432-2446 (2019)
  9. Structural monitoring of a transient intermediate in the hemagglutinin fusion machinery on influenza virions. Benhaim MA, Mangala Prasad V, Garcia NK, Guttman M, Lee KK. Sci Adv 6 eaaz8822 (2020)
  10. Concentration-Dependent Structural Transition of the HIV-1 gp41 MPER Peptide into α-Helical Trimers. Chiliveri SC, Louis JM, Bax A. Angew Chem Int Ed Engl 60 166-170 (2021)
  11. Harnessing low dimensionality to visualize the antibody-virus landscape for influenza. Einav T, Creanga A, Andrews SF, McDermott AB, Kanekiyo M. Nat Comput Sci 3 164-173 (2023)
  12. Investigating host-virus interaction mechanism and phylogenetic analysis of viral proteins involved in the pathogenesis. Naqvi AAT, Anjum F, Shafie A, Badar S, Elasbali AM, Yadav DK, Hassan MI. PLoS One 16 e0261497 (2021)
  13. Laser-Fabricated 2D Molybdenum Disulfide Electronic Sensor Arrays for Rapid, Low-Cost, Ultrasensitive Detection of Influenza A and SARS-Cov-2. Muratore C, Muratore MK, Austin DR, Miesle P, Benton AK, Beagle LK, Motala MJ, Moore DC, Slocik JM, Brothers MC, Kim SS, Krupa K, Back TA, Grant JT, Glavin NR. Adv Mater Interfaces 9 2102209 (2022)
  14. A pooled genome-wide screening strategy to identify and rank influenza host restriction factors in cell-based vaccine production platforms. Sharon DM, Nesdoly S, Yang HJ, Gélinas JF, Xia Y, Ansorge S, Kamen AA. Sci Rep 10 12166 (2020)
  15. Analysis of Interaction Between Odorant Receptors and Flexible Spike of SARS CoV-2- Key to Loss of Smell. Anwar F, Altayeb H, Alhayyani S, Kumar V, Al-Abbasi FA, Ashraf GM. Curr Neuropharmacol 21 151-159 (2023)
  16. Breathing and Tilting: Mesoscale Simulations Illuminate Influenza Glycoprotein Vulnerabilities. Casalino L, Seitz C, Lederhofer J, Tsybovsky Y, Wilson IA, Kanekiyo M, Amaro RE. ACS Cent Sci 8 1646-1663 (2022)
  17. Chimeric Virus-like Particles Co-Displaying Hemagglutinin Stem and the C-Terminal Fragment of DnaK Confer Heterologous Influenza Protection in Mice. Liu CC, Liu DJ, Yue XY, Zhong XQ, Wu X, Chang HY, Wang BZ, Wan MY, Deng L. Viruses 14 2109 (2022)
  18. Cholesterol Binding to the Transmembrane Region of a Group 2 Hemagglutinin (HA) of Influenza Virus Is Essential for Virus Replication, Affecting both Virus Assembly and HA Fusion Activity. Hu B, Höfer CT, Thiele C, Veit M. J. Virol. 93 (2019)
  19. Efficient expression, purification, and visualization by cryo-EM of unliganded near full-length HER3. Diwanji D, Trenker R, Jura N, Verba KA. Methods Enzymol 667 611-632 (2022)
  20. Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection. Del Rosario JMM, da Costa KAS, Asbach B, Ferrara F, Ferrari M, Wells DA, Mann GS, Ameh VO, Sabeta CT, Banyard AC, Kinsley R, Scott SD, Wagner R, Heeney JL, Carnell GW, Temperton NJ. Vaccines (Basel) 9 741 (2021)
  21. HIV-1 Envelope and MPER Antibody Structures in Lipid Assemblies. Rantalainen K, Berndsen ZT, Antanasijevic A, Schiffner T, Zhang X, Lee WH, Torres JL, Zhang L, Irimia A, Copps J, Zhou KH, Kwon YD, Law WH, Schramm CA, Verardi R, Krebs SJ, Kwong PD, Doria-Rose NA, Wilson IA, Zwick MB, Yates JR, Schief WR, Ward AB. Cell Rep 31 107583 (2020)
  22. Host Cell Membrane Capture by the SARS-CoV-2 Spike Protein Fusion Intermediate. Su R, Zeng J, Marcink TC, Porotto M, Moscona A, O'Shaughnessy B. ACS Cent Sci 9 1213-1228 (2023)
  23. Improved epitope resolution of the prefusion trimer-specific antibody AM14 bound to the RSV F glycoprotein. Harshbarger W, Abeyrathne PD, Tian S, Huang Y, Chandramouli S, Bottomley MJ, Malito E. MAbs 13 1955812 (2021)
  24. Inadequate structural constraint on Fab approach rather than paratope elicitation limits HIV-1 MPER vaccine utility. Tan K, Chen J, Kaku Y, Wang Y, Donius L, Khan RA, Li X, Richter H, Seaman MS, Walz T, Hwang W, Reinherz EL, Kim M. Nat Commun 14 7218 (2023)
  25. Influenza A H1 and H3 Transmembrane Domains Interact Differently with Each Other and with Surrounding Membrane Lipids. Kubiszewski-Jakubiak S, Worch R. Viruses 12 (2020)
  26. Librator: a platform for the optimized analysis, design, and expression of mutable influenza viral antigens. Li L, Changrob S, Fu Y, Stovicek O, Guthmiller JJ, McGrath JJC, Dugan HL, Stamper CT, Zheng NY, Huang M, Wilson PC. Brief Bioinform 23 bbac028 (2022)
  27. Membrane-Bound Configuration and Lipid Perturbing Effects of Hemagglutinin Subunit 2 N-Terminus Investigated by Computer Simulations. Michalski M, Setny P. Front Mol Biosci 9 826366 (2022)
  28. Molecular Mechanisms behind Conformational Transitions of the Influenza Virus Hemagglutinin Membrane Anchor. Michalski M, Setny P. J Phys Chem B 127 9450-9460 (2023)
  29. Multiple conformations of trimeric spikes visualized on a non-enveloped virus. Zhang Y, Cui Y, Sun J, Zhou ZH. Nat Commun 13 550 (2022)
  30. Palmitoylation of the hemagglutinin of influenza B virus by ER-localized DHHC enzymes 1, 2, 4, and 6 is required for efficient virus replication. Meng X, Veit M. J Virol e0124523 (2023)
  31. Peptide Models of the Cytoplasmic Tail of Influenza A/H1N1 Virus Hemagglutinin Expand Understanding its pH-Dependent Modes of Interaction with Matrix Protein M1. Poboinev VV, Khrustalev VV, Akunevich AA, Shalygo NV, Stojarov AN, Khrustaleva TA, Kordyukova LV. Protein J (2023)
  32. Purification and characterization of the Lassa virus transmembrane domain. Keating PM, Pennington HN, Collins SD, Lee J. Biochem Biophys Rep 33 101409 (2023)
  33. Reversible structural changes in the influenza hemagglutinin precursor at membrane fusion pH. Garcia-Moro E, Zhang J, Calder LJ, Brown NR, Gamblin SJ, Skehel JJ, Rosenthal PB. Proc Natl Acad Sci U S A 119 e2208011119 (2022)
  34. Rotational Dynamics of The Transmembrane Domains Play an Important Role in Peptide Dynamics of Viral Fusion and Ion Channel Forming Proteins-A Molecular Dynamics Simulation Study. Wang CW, Fischer WB. Viruses 14 699 (2022)
  35. Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycans. Chmielewski D, Wilson EA, Pintilie G, Zhao P, Chen M, Schmid MF, Simmons G, Wells L, Jin J, Singharoy A, Chiu W. Nat Commun 14 7175 (2023)
  36. Temporal and Gene Reassortment Analysis of Influenza C Virus Outbreaks in Hong Kong, SAR, China. Daniels RS, Galiano M, Ermetal B, Kwong J, Lau CS, Xiang Z, McCauley JW, Lo J. J Virol 96 e0192821 (2022)
  37. The Pre-Existing Human Antibody Repertoire to Computationally Optimized Influenza H1 Hemagglutinin Vaccines. Nagashima K, Dzimianski JV, Han J, Abbadi N, Gingerich AD, Royer F, O'Rourke S, Sautto GA, Ross TM, Ward AB, DuBois RM, Mousa JJ. J Immunol 209 5-15 (2022)
  38. Topological analysis of the gp41 MPER on lipid bilayers relevant to the metastable HIV-1 envelope prefusion state. Wang Y, Kaur P, Sun ZJ, Elbahnasawy MA, Hayati Z, Qiao ZS, Bui NN, Chile C, Nasr ML, Wagner G, Wang JH, Song L, Reinherz EL, Kim M. Proc. Natl. Acad. Sci. U.S.A. 116 22556-22566 (2019)
  39. Unique properties of Coronaviridae single-pass transmembrane domain regions as an adaptation to diverse membrane systems. Kubiszewski-Jakubiak S, Worch R. Virology 570 1-8 (2022)
  40. iBRAB: In silico based-designed broad-spectrum Fab against H1N1 influenza A virus. Do PC, Nguyen TH, Vo UHM, Le L. PLoS One 15 e0239112 (2020)
  41. saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern. Komori M, Nogimori T, Morey AL, Sekida T, Ishimoto K, Hassett MR, Masuta Y, Ode H, Tamura T, Suzuki R, Alexander J, Kido Y, Matsuda K, Fukuhara T, Iwatani Y, Yamamoto T, Smith JF, Akahata W. Nat Commun 14 2810 (2023)


Quick links